Overview

  • Product nameAnti-Gemin 3 antibody
    See all Gemin 3 primary antibodies
  • Description
    Mouse polyclonal to Gemin 3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Gemin 3 protein (AAH34953.1).

  • Positive control
    • Human placenta tissue lysate, Gemin 3 transfected 293T cell lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX20 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationCytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • Component of gems 3 antibody
    • DDX 20 antibody
    • ddx20 antibody
    • DDX20_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
    • DEAD box protein 20 antibody
    • DEAD box protein DP 103 antibody
    • DEAD box protein DP103 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
    • DEAD/H BOX 20 antibody
    • DKFZP434H052 antibody
    • DP 103 antibody
    • DP103 antibody
    • Gemin-3 antibody
    • Gemin3 antibody
    • Probable ATP dependent RNA helicase DDX20 antibody
    • Probable ATP-dependent RNA helicase ddx20 antibody
    • SMN interacting protein antibody
    see all

Anti-Gemin 3 antibody images

  • Anti-Gemin 3 antibody (ab88863) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 125 kDa,250 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Gemin 3 antibody (ab88863) at 1 µg/ml

    Lane 1 : Gemin 3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 92 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • ab88863, at 10 µg/ml staining Gemin 3 in HeLa cells by Immunofluorescence.

References for Anti-Gemin 3 antibody (ab88863)

ab88863 has not yet been referenced specifically in any publications.

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