Recombinant
RabMAb

Anti-Gemin 3 antibody [EPR11283] (ab166913)

Overview

  • Product name
    Anti-Gemin 3 antibody [EPR11283]
    See all Gemin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11283] to Gemin 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Gemin 3. The exact sequence is proprietary.

  • Positive control
    • HeLa, MCF-7 and 293T cell lysate. Human colon tissue. Human kidney tissue. Permeabilized 293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250

Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX20 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization
      Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • Component of gems 3 antibody
      • DDX 20 antibody
      • ddx20 antibody
      • DDX20_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
      • DEAD box protein 20 antibody
      • DEAD box protein DP 103 antibody
      • DEAD box protein DP103 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
      • DEAD/H BOX 20 antibody
      • DKFZP434H052 antibody
      • DP 103 antibody
      • DP103 antibody
      • Gemin-3 antibody
      • Gemin3 antibody
      • Probable ATP dependent RNA helicase DDX20 antibody
      • Probable ATP-dependent RNA helicase ddx20 antibody
      • SMN interacting protein antibody
      see all

    Images

    • All lanes : Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 92 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).

    References

    ab166913 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab166913.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up