Anti-Gemin 3 antibody [EPR11283] (ab166913)


  • Product name
    Anti-Gemin 3 antibody [EPR11283]
    See all Gemin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11283] to Gemin 3
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Gemin 3.

  • Positive control
    • HeLa, MCF-7 and 293T cell lysate. Human colon tissue. Human kidney tissue. Permeabilized 293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab166913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250

Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
    • Tissue specificity
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX20 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Cellular localization
      Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • Component of gems 3 antibody
      • DDX 20 antibody
      • ddx20 antibody
      • DDX20_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
      • DEAD box protein 20 antibody
      • DEAD box protein DP 103 antibody
      • DEAD box protein DP103 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
      • DEAD/H BOX 20 antibody
      • DKFZP434H052 antibody
      • DP 103 antibody
      • DP103 antibody
      • Gemin-3 antibody
      • Gemin3 antibody
      • Probable ATP dependent RNA helicase DDX20 antibody
      • Probable ATP-dependent RNA helicase ddx20 antibody
      • SMN interacting protein antibody
      see all


    • All lanes : Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 92 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).


    ab166913 has not yet been referenced specifically in any publications.

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