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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
Use a concentration of 5 µg/ml.
Anti-Gemin 4 antibody images
Western blot - Anti-Gemin 4 antibody (ab31581)
All lanes :
Anti-Gemin 4 antibody (ab31581) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat whole cell lysate ( ab7899) Lane 3 : A431 whole cell lysate ( ab7909) Lane 4 : HEK293 whole cell lysate ( ab7902) Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size : 120 kDa Observed band size : 110 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 4 antibody (ab31581)
ICC/IF image of ab31581 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31581, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-Gemin 4 antibody (ab31581)
This product has been referenced in:
et al. Nuclear repartitioning of galectin-1 by an extracellular glycan switch regulates mammary morphogenesis. Proc Natl Acad Sci U S A 113:E4820-7 (2016).
Read more (PubMed: 27496330) »
et al. Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis. Mol Cell Proteomics 15:1220-31 (2016).
Read more (PubMed: 26711467) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"