Overview

  • Product name
    Anti-Gemin 4 antibody
    See all Gemin 4 primary antibodies
  • Description
    Rabbit polyclonal to Gemin 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1058) of Human Gemin 4 (NP_056536.1).

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 120 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    GEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus.
  • Cellular localization
    Cytoplasmic and nuclear.
  • Database links
    • Alternative names
      • Component of gems 4 antibody
      • Gem associated protein 4 antibody
      • HC56 antibody
      • HHRF 1 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 4 antibody (ab70750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 4 antibody (ab70750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
    • Western blot - Gemin 4 antibody (ab70750)
      Western blot - Gemin 4 antibody (ab70750)
      All lanes : Anti-Gemin 4 antibody (ab70750) at 1/5000 dilution

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 120 kDa
      Observed band size: 117 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 160 kDa, 270 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Immunoprecipitation - Gemin 4 antibody (ab70750)
      Immunoprecipitation - Gemin 4 antibody (ab70750)
      Detection of Gemin 4 by Western Blot of Immunprecipitate. ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

    References

    ab70750 has not yet been referenced specifically in any publications.

    Publishing research using ab70750? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Gemin 4 antibody

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK-293)
    Permeabilization
    Yes - 0.3% Triton X-100 in Blocking Buffer
    Specification
    HEK-293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Mar 22 2018

    Western blot abreview for Anti-Gemin 4 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocyte)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-tris)
    Loading amount
    40 µg
    Specification
    Lymphocyte
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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