Overview

  • Product nameAnti-Gemin 5 antibody
    See all Gemin 5 primary antibodies
  • Description
    Rabbit polyclonal to Gemin 5
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    A region within amino acids 1360-1410 of Human Gemin 5 (NP_056280.1)

  • Positive control
    • HeLa whole cell lysate. 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99360 was affinity purified using an epitope specific to Gemin 5 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab99360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceGemin 5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2 (SIP1), Gemin 3 (DDX20), and Gemin 4.
  • Cellular localizationNuclear and cytoplasmic
  • Database links
  • Alternative names
    • gem (nuclear organelle) associated protein 5 antibody
    • Gem-associated protein 5 antibody
    • GEMIN5 antibody

Anti-Gemin 5 antibody images

  • All lanes : Anti-Gemin 5 antibody (ab99360) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 169 kDa


    Exposure time : 10 seconds
  • ab99360 at 1 µg/ml detecting Gemin 5 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
    For immunoprecipitation, ab99360 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99360
    Lane 2; IP using control IgG.

References for Anti-Gemin 5 antibody (ab99360)

ab99360 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"