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Recombinant fragment, corresponding to amino acids 110-209 of Human Geminin (AAH05185) with a proprietary tag.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab195047 as a replacement.
Our Abpromise guarantee covers the use of ab104306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Detection limit for recombinant tagged Geminin is approximately 0.03ng/ml when used as a capture antibody.|
|IHC-P||Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"