Overview

  • Product name
  • Description
    Rabbit polyclonal to Geminin
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide: KRKHRNDHLT STTSSPGVIV PESSENKNLG GVTQESFDLM IKENPSSQYW K, corresponding to amino acids 50-100 of Human Geminin (NP_056979.1)

  • Positive control
    • Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer recommended.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
    • Sequence similarities
      Belongs to the geminin family.
    • Developmental stage
      Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA replication inhibitor antibody
      • Gem antibody
      • GEMI_HUMAN antibody
      • Geminin antibody
      • Geminin DNA replication inhibitor antibody
      • GMNN antibody
      • OTTHUMP00000039393 antibody
      • RP3 369A17.3 antibody
      see all

    Images

    • ab84423 staining Geminin in Human tonsil tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in EDTA pH8.0 for 20 minutes at 100°C. Samples were incubated with primary antibody (1/40) for 20 minutes at 25°C. A HPR-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84423, followed by DAB staining.

    References

    ab84423 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Specification
    tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH8.0, 20 min, 100C
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    Submitted May 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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