Overview

  • Product nameGenomic DNA Isolation Kit
  • Tests
    50 x 1 assay
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    2h 00m
  • Product overview

    Abcam's Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA. The extracted genomic DNA is free from protein and RNA, and suitable for a variety of applications such as PCR, DNA hybridization, enzyme manipulation, cloning, Southern blot, and array-based experiments.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsSuitable for: Purificationmore details

Properties

  • RelevanceGenomic DNA is the full complement of nuclear DNA contained in the cell or organism genome.
  • Alternative names
    • DNA

Associated products

Applications

Our Abpromise guarantee covers the use of ab65358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Protocols

References for Genomic DNA Isolation Kit (ab65358)

This product has been referenced in:
  • Marrache S  et al. Detouring of cisplatin to access mitochondrial genome for overcoming resistance. Proc Natl Acad Sci U S A 111:10444-9 (2014). Read more (PubMed: 25002500) »
  • Esmaeili F  et al. Stable suppression of gene expression by short interfering RNAs targeted to promoter in a mouse embryonal carcinoma stem cell line. In Vitro Cell Dev Biol Anim 46:834-40 (2010). Southern Blot . Read more (PubMed: 20872183) »

See all 2 Publications for this product

Product Wall

We always provide more material than necessary which sometimes results in a super saturated solution. This can lead to a hazy solution. Typically this does not compromise function in any way. You can warm the solution up to 37C or use mild sonication i...

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Thank you for your enquiry and your interest in our products.

This kit ab65353 is a DNA Damage Quantification Kit which is to be used with purified DNA. You cannot use frozen tissues directly with this kit. The frozen tissue will need pre-pro...

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Thank you for your patient. As this kit is meant to isolate genomic DNA, there is no data for cytoplasmic DNA, and therefore I cannot confirm its suitability for it. I am sorry I couldn’t be more helpful. Please do not hesitate to contact us if you nee...

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Thanks for your call last week and for your patience while I have been in touch with the production lab regarding ab65358. I apologize for the delay in getting back to you. This kit will isolate the total genomic DNA, however the minimum/ maximum lengt...

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Thank you for your inquiry. Yes, the customer should be able to use this kit. “For tissue samples, ground freshly excised tissue in liquid nitrogen. Weigh ~5 mg grounded fine tissue powder in a microcentrifuge tube.” The rest of the steps can be fol...

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Thank you for your inquiry.

This kit has not been tested with dental plaque samples.

To my knowledge dental plaque consists mainly of bacteria.

Since this kit applied a extraction of the nucleus and then goes on to extract...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"