Overview

  • Product name
  • Description
    Rabbit polyclonal to Gephyrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human Gephyrin aa 250-300. The exact sequence is proprietary. NP_001019389.1.
    Sequence:

    SRGVQVLPRDT ASLSTTPSES PRAQATSRLS TASCPTPKVQ SRCSSKENIL


    Database link: Q9NQX3

  • Positive control
    • HeLa, 293T and Jurkat cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab185993 was affinity purified using an epitope specific to Gephyrin immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
  • Pathway
    Cofactor biosynthesis; molybdopterin biosynthesis.
  • Involvement in disease
    Defects in GPHN are the cause of molybdenum cofactor deficiency type C (MOCOD type C) [MIM:252150]. MOCOD type C is an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.
    Defects in GPHN are a cause of startle disease (STHE) [MIM:149400]; also known as hyperekplexia. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • Sequence similarities
    In the N-terminal section; belongs to the moaB/mog family.
    In the C-terminal section; belongs to the moeA family.
  • Cellular localization
    Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Cytoplasmic face of glycinergic postsynaptic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Domain E antibody
    • Domain G antibody
    • GEPH antibody
    • GEPH_HUMAN antibody
    • GPH antibody
    • GPHN antibody
    • GPHRYN antibody
    • KIAA1385 antibody
    • Molybdopterin molybdenumtransferase antibody
    • MPT adenylyltransferase antibody
    • MPT Mo-transferase antibody
    see all

Images

  • Detection of Gephyrin in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185993 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab185993 was used at 1µg/ml. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • All lanes : Anti-Gephyrin antibody (ab185993) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 80 kDa


    Exposure time: 3 minutes

References

ab185993 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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