Anti-Germ cell-specific antigen antibody [TRA98] (ab82527)

Overview

  • Product nameAnti-Germ cell-specific antigen antibody [TRA98]
  • Description
    Rat monoclonal [TRA98] to Germ cell-specific antigen
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Germ cell-specific antigen. Cell lysates of adult mouse testes.

  • Positive control
    • Testicular germ cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab82527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent dilution.
WB 1/5000. Predicted molecular weight: 146 kDa.

Target

  • RelevanceDetected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
  • Alternative names
    • GENA antibody
    • Testicular germ cell-specific antigen antibody

Anti-Germ cell-specific antigen antibody [TRA98] images

  • ab82527 staining Germ cell-specific antigen in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 3% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C. Ab150165 was used as the secondary antibody, undiluted.

  • ab82527 staining Germ cell-specific antigen in mouse postnatal day 7 testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 45 minutes at 25°C; antigen retrieval was by heat mediation in 0.1M citric acid/sodium citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA) for 1 hour at 25°C. A biotin-conjugated goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Germ cell-specific antigen antibody [TRA98] (ab82527) at 0.1 µg/ml

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 3 : mouse intestine lysate
    Lane 4 : mouse kidney lysate
    Lane 5 : mouse liver lysate
    Lane 6 : mouse lung lysate
    Lane 7 : mouse spleen lysate
    Lane 8 : mouse ovary lysate
    Lane 9 : mouse testis lysate

    Lysates/proteins at 100 µg per lane.


    Predicted band size : 146 kDa
    Western blot analysis of various mouse tissues, using ab82527.
    In adult tissues, bands of 60-100 kDa proteins are detected only in the testis. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells.
  • Immunohistochemical analysis of mouse fetal ovary tissue, staining Germ cell-specific antigen with ab82527.

    Tissue was fixed with Bouin's fixative, permeabilized with 0.05% Tween 20 and 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA and 0.5% Triton X-100 in 1X PBS) for 1 hour at 25°C. An AlexaFluor®488-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Germ cell-specific antigen antibody [TRA98] (ab82527)

This product has been referenced in:
  • Lim J  et al. The Mouse Fetal Ovary Has Greater Sensitivity Than the Fetal Testis to Benzo[a]pyrene-Induced Germ Cell Death. Toxicol Sci 152:372-81 (2016). Read more (PubMed: 27208085) »
  • Pellegrino J  et al. Chromatin associated Sin3A is essential for male germ cell lineage in the mouse. Dev Biol 369:349-55 (2012). IHC-P ; Mouse . Read more (PubMed: 22820070) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, adult)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, adult
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Apr 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (fibroblast)
Permeabilization No
Specification fibroblast
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative ice-cold acetone
Username

Abcam user community

Verified customer

Submitted Jul 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
Sample Mouse Tissue sections (Postnatal day 7 mouse testes)
Specification Postnatal day 7 mouse testes
Permeabilization No
Fixative 4% PFA
Username

Abcam user community

Verified customer

Submitted Dec 29 2014

Thank you for your inquiry and your patience with this reply.
For ab82527, it should be IgG2a, although we do not do any validation to verify that. The clone TRA98 recognizes a mouse testicular germ cell-specific antigen (1). In adult tissues, ban...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (fetal ovary)
Specification fetal ovary
Fixative Bouin's fixative
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization Yes - 0.05% Tween 20 and 0.1% Triton X-100 in blocking serum
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 06 2012

DISCOUNT CODE: ***
Expiration date: February 15th, 2012
Value: ***

I am very pleased to hear you would like to accept our offer and test ab82527 in IHC-P. This code will give you *** off your next order before the expiration date. To...

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Thank you very much for your email.
I am happy to arrange a credit note or refund. However I would need more information about the problem customer experienced. Our customers feedback and own tested data show that the antibody detects Germ cells v...

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Thank you very much for your valuable feedback.

We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us.

To find...

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Thank you for contacting us. To answer your questions: 1) Yes, JLP is the same as SPAG9. For more alternative names, please check the protein database entry for the mouse protein: http://www.uniprot.org/uniprot/Q58A65 2)References are listed in the ref...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"