Anti-Germinal Center Kinase antibody (ab82870)

Overview

  • Product nameAnti-Germinal Center Kinase antibody
    See all Germinal Center Kinase primary antibodies
  • Description
    Rabbit polyclonal to Germinal Center Kinase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 216-265 (HLHPMRALML MSKSSFQPPK LRDKTRWTQN FHHFLKLALT KNPKKRPTAE) of human Germinal Center Kinase (NP_004570).

  • Positive control
    • Fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionEnhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
  • Tissue specificityHighly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Basolateral cell membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphocyte serine/threonine protein kinase antibody
    • B lymphocyte serine/threonine-protein kinase antibody
    • BL44 antibody
    • GC kinase antibody
    • GCK antibody
    • Germinal center kinase antibody
    • germinal centre kinase (GC kinase) antibody
    • M4K2_HUMAN antibody
    • Map4k2 antibody
    • MAPK/ERK kinase kinase kinase 2 antibody
    • MEK kinase kinase 2 antibody
    • MEKKK 2 antibody
    • Mitogen activated protein kinase kinase kinase kinase 2 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 2 antibody
    • Rab8 interacting protein antibody
    • Rab8 interacting protein, formerly antibody
    • Rab8-interacting protein antibody
    • RAB8IP antibody
    • RAB8IP, formerly antibody
    see all

Anti-Germinal Center Kinase antibody images

  • Anti-Germinal Center Kinase antibody (ab82870) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 92 kDa
    Observed band size : 89 kDa (why is the actual band size different from the predicted?)

References for Anti-Germinal Center Kinase antibody (ab82870)

ab82870 has not yet been referenced specifically in any publications.

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