Overview

  • Product nameAnti-GERP antibody
    See all GERP primary antibodies
  • Description
    Rabbit polyclonal to GERP
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: RGCIGEAWAK DSGLVRCPEC NQAYNQKPGL EKNLKLTNIV EKFNALHVEK , corresponding to N terminal amino acids 35-84 of Human GERP (NP_112174)

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionProbable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1.
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • DomainThe coiled coil domain is required for homodimerization.
    The region immediately C-terminal to the RING motif is sufficient to mediate the interaction with SOCS1.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA408830 antibody
    • BB006755 antibody
    • Glioblastoma-expressed RING finger protein antibody
    • Lioblastoma expressed ring finger protein antibody
    • Probable E3 ubiquitin-protein ligase TRIM8 antibody
    • RING finger protein 27 antibody
    • RNF27 antibody
    • RP11-47A8.4 antibody
    • TRIM8 antibody
    • TRIM8_HUMAN antibody
    • Tripartite motif containing 8 antibody
    • Tripartite motif protein TRIM8 antibody
    • Tripartite motif-containing protein 8 antibody
    see all

Anti-GERP antibody images

  • Anti-GERP antibody (ab83395) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GERP with ab83395 at 5.0µg/ml.

References for Anti-GERP antibody (ab83395)

ab83395 has not yet been referenced specifically in any publications.

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