Biological descriptionPotent, cell-permeable, ATP-competitive PKC inhibitor (IC50 values for PKC subtypes are 50 (α), 17 (βI), 16 (βII) and 20 nM (γ)). Inhibits PKC over other kinases (IC50 values are 50 nM (MAPKAP), 100 nM (p70 S6 kinase), 2 μM (cAMP-dependent protein kinase) and 0.7 μM (phosphorylase kinase)). Crosses the blood-brain barrier.
Chemical name3-[1-(Dimethylaminopropyl)indol-3-yl]-4-(indol-3-yl)maleimide hydrochloride
Storage instructionsStore at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
References for GF 109203X hydrochloride (ab120844)
This product has been referenced in:
Cheung HH et al. Inhibition of protein kinase C reduces ischemia-induced tyrosine phosphorylation of the N-methyl-d-aspartate receptor. J Neurochem86:1441-9 (2003).
Read more (PubMed: 12950452) »
Alessi DR The protein kinase C inhibitors Ro 318220 and GF 109203X are equally potent inhibitors of MAPKAP kinase-1beta (Rsk-2) and p70 S6 kinase. FEBS Lett402:121-3 (1997).
Read more (PubMed: 9037179) »
Toullec D et al. The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem266:15771-81 (1991).
Read more (PubMed: 1874734) »