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Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
This clone gives a much stronger signal in pig, cow and human samples than in rodents. For rodent samples use ab68428.
Our Abpromise guarantee covers the use of ab4648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use this antibody as Capture antibody with Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) as Detection antibody at 0.5µg/ml.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||1/10 - 1/50.|
|WB||1/100 - 1/500. Detects a band of approximately 55 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
Standard Curve for GFAP; dilution range 1 pg/ml to 1 µg/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 1/5000 and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 µg/ml.