Anti-GFAP antibody [2A5] - Astrocyte Marker (ab4648)
- Product nameAnti-GFAP antibody [2A5] - Astrocyte MarkerSee all GFAP primary antibodies ...
- DescriptionMouse monoclonal [2A5] to GFAP - Astrocyte Marker
- Tested applicationsSandwich ELISA, ICC, IHC-P, ICC/IF, WB, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
Predicted to work with: all Mammals
Does not react withChicken
A preparation of purified pig spinal core GFAP.
- Positive controlHomogenized cow, pig, human, rat, mouse or other mammalian brain extract in 1%SDS, 6M urea.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 10mM Sodium Azide
Constituents: Concentrated Tissue Culture Supernatant
- PurityTissue culture supernatant
- Purification notesAntibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant.
- Clonality Monoclonal
- Clone number2A5
- Light chain typekappa
Our Abpromise guarantee covers the use of ab4648 in the following tested applications.
|Sandwich ELISA||sELISA: Use a concentration of 0.2 µg/ml. Use this antibody as Capture antibody with Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) as Detection antibody at 0.5µg/ml.|
|ICC||ICC: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: 1/10 - 1/50.|
|WB||WB: 1/100 - 1/500. Detects a band of approximately 55 kDa.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
- FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Tissue specificityExpressed in cells lacking fibronectin.
- Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
- Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylated by PKN1.
- Cellular localizationCytoplasm. Associated with intermediate filaments.
- Astrocyte antibodyFLJ42474 antibodyFLJ45472 antibody
- GFAP antibodyGFAP_HUMAN antibodyGlial Fibrillary Acidic Protein antibodyIntermediate filament protein antibody
Anti-GFAP antibody [2A5] - Astrocyte Marker images
Standard Curve for GFAP; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 ug/ml.
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
Rat neuron/glia cultures stained with nmouse monoclonal to GFAP ab 4648 (red).
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab4648 staining GFAP - Astrocyte Marker in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody 1/2000 (TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to mouse IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.
References for Anti-GFAP antibody [2A5] - Astrocyte Marker (ab4648)
This product has been referenced in:
- Hao W et al. Myeloid differentiation factor 88-deficient bone marrow cells improve Alzheimer's disease-related symptoms and pathology. Brain 134:278-92 (2011). ICC/IF ; Mouse . Read more (PubMed: 21115468) »
- Splinter E et al. The inactive X chromosome adopts a unique three-dimensional conformation that is dependent on Xist RNA. Genes Dev 25:1371-83 (2011). Read more (PubMed: 21690198) »