Overview

  • Product name
  • Description
    Rabbit polyclonal to GFAP
  • Tested applications
    Suitable for: IHC-Fr, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
  • Immunogen

    Full length native protein (purified) (Cow). Glial fibrillary protein isolated from cow spinal cord

  • Positive control
    • Brain
  • General notes


    In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24223835
ICC 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 20460993
IHC-P 1/50 - 1/250.

Target

  • Function
    GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificity
    Expressed in cells lacking fibronectin.
  • Involvement in disease
    Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localization
    Cytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Images

  • ab16997 (1/100) staining astrocytes in mouse cortical primary cell culture.

  • Formalin-fixed, paraffin-embedded section of brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, staining GFAP with ab16997.

References

This product has been referenced in:
  • Getz AM  et al. Tumor suppressor menin is required for subunit-specific nAChR a5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons. Sci Rep 7:1768 (2017). ICC ; Mouse . Read more (PubMed: 28496137) »
  • Li Y  et al. Age-related changes in hypertensive brain damage in the hippocampi of spontaneously hypertensive rats. Mol Med Rep 13:2552-60 (2016). IHC . Read more (PubMed: 26846626) »

See all 17 Publications for this product

Customer reviews and Q&As

After completing a short literature search, GFAP is commonly used as an protoplasmic astrocyte marker. Below are two references:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038243
http://www.jneurosci.org/content...

Read More

I can confirm that ab16997 will only detect GFAP in astorcytes not in oligodendrocytes and micogliocytes (these cells do not express this protein).

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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