Overview

  • Product nameAnti-GFAP antibody
    See all GFAP primary antibodies
  • Description
    Chicken polyclonal to GFAP
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Human, Rhesus monkey, Apteronotus leptorhynchus
    Predicted to work with: all Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Cow GFAP.

  • Positive control
    • IHC-P: FFPE mouse brain normal. IHC-P: FFPE rat hippocampus normal.

Properties

Applications

Our Abpromise guarantee covers the use of ab4674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-Fr 1/1000 - 1/5000. Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.
IHC-FoFr 1/1000 - 1/5000. PubMed: 20098733Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.

Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.

ICC/IF 1/1000 - 1/5000.

Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.

PubMed: 25418722

Target

  • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificityExpressed in cells lacking fibronectin.
  • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localizationCytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody images

  • IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat hippocampus tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

  • IHC image of GFAP staining in a formalin fixed, paraffin embedded mouse normal brain tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

  • Anti-GFAP antibody (ab4674) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg

    Secondary
    AlexaFluor®488-conjugated goat anti-chicken polyclonal IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab4674 staining GFAP in mouse hippocampus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocking with 10% serum for 30 minutes at 250C was performed. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. Staining was intensified with 2-3 minutes of retrieval with trypsin at room temperature.

    See Abreview

  • Rat astrocytes stained with ab4674 (red). The anti-GFAP antibody was used at a dilution of 1:50 from affinity purified material using our standard fixation and staining procedure (in protocol section). Hoechst dye reveals nuclear DNA in blue.
  • ab4674 staining GFAP in rat primary astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 20 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 24 hours at 4°C. ab6873, a goat anti-chicken IgY FITC (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GFAP antibody (ab4674) at 1/5000 dilution

    Lane 1 : Whole rat brain extract
    Lane 2 : Mouse brain extract


    Predicted band size : 50 kDa
  • ab4674 staining GFAP in Rabbit eye tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with BSA for 2 hours at 4°C. The sample was incubated with primary antibody (1/1000) at 4°C for 12 hours. ab150175, a goat anti-chicken IgY Alexa Fluor® 647 (1/1000), was used as the secondary antibody.

    See Abreview

  • ab4674 staining GFAP - Astrocyte Marker in Human Brain cells by Flow Cytometry. Cells were grown in stem cell media, RHB-A, and collected using 0.05% trypsin + trypsin inhibitor. Cells were fixed in 80% Acetone for 5-10 minutes at -20°C. The sample was incubated with the primary antibody (1/100 in 10% FCS in PBS) for 20 minutes at 25°C. ab46969 a goat anti-chicken IgY FITC (1/100) was used as the secondary antibody
    Gating Strategy: Cells expressing GFAP

    See Abreview

  • ab4674 detecting recombinant GFAP-Astrocyte Marker by direct ELISA. Mouose recombinat GFAP Protein was coated onto a microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C. Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An undiluted alkaline phosphatase Goat anti-chicken IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-GFAP antibody (ab4674)

This product has been referenced in:

See all 58 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 4°C
Antigen retrieval step None
Sample Rabbit Tissue sections (eye)
Specification eye
Permeabilization No
Fixative Formaldehyde
Username

Dr. Alex Zagariya

Verified customer

Submitted Dec 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (Olfactory stem cells)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Olfactory stem cells GFP)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells GFP
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Olfactory stem cells)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Olfactory stem cells (neuronal indicution))
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells (neuronal indicution)
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Sep 20 2016

Application IHC - Wholemount
Sample Mouse Tissue (Colon)
Specification Colon
Username

Abcam user community

Verified customer

Submitted Jun 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - 50% ethanol, 15min
Specification Spinal cord
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 11 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Mouse hippocampus)
Specification Mouse hippocampus
Permeabilization Yes - 0.2% Triton X100
Fixative Paraformaldehyde
Username

gergely kovacs

Verified customer

Submitted Mar 18 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Scytek blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Rat Cell (Rat primary astrocytes)
Specification Rat primary astrocytes
Permeabilization No
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Jan 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 10 2014

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