Overview

  • Product nameAnti-GFAP antibody
    See all GFAP primary antibodies
  • Description
    Goat polyclonal to GFAP
  • Tested applicationsSuitable for: IHC-FrFl, WB, Sandwich ELISA, IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human GFAP aa 417-430 (C terminal) (Cysteine residue).
    Sequence:

    DGEVIKESKQEHKD

  • Positive control
    • WB: Mouse brain tissue lysate. IHC-P: Human cerebellum tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/500 - 1/2000.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21943601

Target

  • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificityExpressed in cells lacking fibronectin.
  • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localizationCytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody images

  • Anti-GFAP antibody (ab53554) at 0.1 µg/ml + Mouse Brain Lysate in RIPA Buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Primary incubation was 1 hour.
  • Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling GFAP with ab53554 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus.

    See Abreview

  • ab53554 staining GFAP in Mongolian gerbil brain tissue sections by Immunohistochemistry - Free Floating.Samples were incubated with ab53554 at a 1/500 dilution in 1% BSA, 0.3% Triton X-100, 0.1% Saponin for 16 hours at 4°C. ab6566 Donkey polyclonal Secondary Antibody to anti-goat Cy5 ® (IgG - H&L)), pre-adsorbed was used as the secondary antibody at a 1/100 dilution (green). Nuclei are counterstained with DAPI.

    See Abreview

  • ab53554 (5ug/ml) as the reporter with a capture rabbit antibody (5ug/ml).

References for Anti-GFAP antibody (ab53554)

This product has been referenced in:
  • Reiner DJ  et al. Astrocytes Regulate GLP-1 Receptor-Mediated Effects on Energy Balance. J Neurosci 36:3531-40 (2016). IF, IHC ; Rat . Read more (PubMed: 27013681) »
  • Cao S  et al. Hydrogen sulfide attenuates brain edema in early brain injury after subarachnoid hemorrhage in rats: Possible involvement of MMP-9 induced blood-brain barrier disruption and AQP4 expression. Neurosci Lett 621:88-97 (2016). IHC ; Rat . Read more (PubMed: 27080433) »

See all 22 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

Submitted Jun 13 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization No
Specification brain
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative Formaldehyde
Username

Miss. Regina Vontell

Verified customer

Submitted May 01 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Zamboni
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 20 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Tissue lysate - other (Frontal Cortex)
Specification Frontal Cortex
Blocking step Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification total larvae, 4 days post fertilization
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Oct 27 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Rat Tissue sections (Nervous Tissue (Retina and Brain))
Specification Nervous Tissue (Retina and Brain)
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Tissue sections (Retina)
Specification Retina
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Mouse Cell (Brain Organotypic Slice)
Specification Brain Organotypic Slice
Permeabilization Yes - 0.3% Triton
Fixative Paraformaldehyde
Username

Ms. Kirsty Ireland

Verified customer

Submitted Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kelly Neuroblastoma Cell line)
Specification Kelly Neuroblastoma Cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 26 2012

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"