Anti-GFAP antibody - Astrocyte Marker (ab16997)

Overview

  • Product nameAnti-GFAP antibody - Astrocyte Marker
    See all GFAP primary antibodies
  • Description
    Rabbit polyclonal to GFAP - Astrocyte Marker
  • Tested applicationsSuitable for: IHC-Fr, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
  • Immunogen

    Full length native protein (purified) (Cow). Glial fibrillary protein isolated from cow spinal cord

  • Positive control
    • Brain
  • General notes


    In the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24223835
ICC 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 20460993
IHC-P 1/50 - 1/250.

Target

  • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificityExpressed in cells lacking fibronectin.
  • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localizationCytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody - Astrocyte Marker images

  • ab16997 (1/100) staining astrocytes in mouse cortical primary cell culture.

  • Formalin-fixed, paraffin-embedded section of brain stained with antibodies to GFAP. Cytoplasmic staining of glia is shown in red.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, staining GFAP with ab16997.

References for Anti-GFAP antibody - Astrocyte Marker (ab16997)

This product has been referenced in:
  • Katayama A  et al. Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis. Sci Rep 5:16920 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26581806) »
  • Lim SH  et al. Neuronal synapse formation induced by microglia and interleukin 10. PLoS One 8:e81218 (2013). WB ; Rat . Read more (PubMed: 24278397) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.

After completing a short literature search, GFAP is commonly used as an protoplasmic astrocyte marker. Below are two references:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00382...

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Thank you for the reply and for providing that information.

I am sorry that the antibody was not working for you and based on the information you have provided, I am not sure if there is anything that I can suggest that will help resolve the i...

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Thank you for contacting Abcam.

You are correct that under our Abpromise, we do guarantee that ab16997 should work in mouse cultures in IF and if we cannot determine the root cause of the issue, then we will happily replace or refund the cost ...

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Thank you for contacting us. I can confirm that ab16997 will only detect GFAP in astorcytes not in oligodendrocytes and micogliocytes (these cells do not express this protein). I hope this information is helpful to you. Please do not hesitate t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step None
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

Submitted Aug 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"