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Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Overview
Product name
Anti-GFAP antibody - Astrocyte Marker
See all GFAP products (35) ...
Description
Rabbit polyclonal to GFAP - Astrocyte Marker
Specificity
This antibody labels the astrocytes in the brain. In peripheral neural system it reacts with Shwann cells, satellite cells, and enteric glial cells. It is positive with astrocytomas in the central neural system. The antibody has been solid-phase absorbed with human and cow serum proteins. In crossed immunoelectrophoresis using 50 µL antibody per cm2 gel area, no reaction with 2 µL human plasma and 2 µL cow serum is observed. The antibody shows one distinct precipitate (GFAP) with cow brain extract. In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
Tested applications
WB, ICC/IF, ICC, IHC-FoFr, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog, Human
Predicted to work with
Sheep, Cat
Immunogen
GFAP isolated from cow spinal cord.
Positive control
brain tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/1000.
IHC-Fo: 1/500 - 1/1000.
IHC-P: 1/500 - 1/1000.
IHC-Fr: 1/500 - 1/1000.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificity
Expressed in cells lacking fibronectin.
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
Phosphorylated by PKN1.
Cellular localization
Cytoplasm. Associated with intermediate filaments.
Target information above from: UniProt accessionP14136
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Astrocyte antibody
- FLJ42474 antibody
- FLJ45472 antibody
see all
Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Entrez Gene: 443220 Sheep
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
see all
Anti-GFAP antibody - Astrocyte Marker images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - GFAP antibody - Astrocyte Marker (ab7779)
ab7779 at a dilution of 1/1000, staining GFAP in astrocytes (Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab7779)
ab7779 staining human brain tissue by ICC/IF. The section was formaldehyde fixed and blocked with 3% serum (for 1 hour at 24°C) prior to incubation with ab7779 (1/1000) for 12 hours at 24°C. An Alexa Fluor® 546 conjugated goat anti-rabbit was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - GFAP antibody - Astrocyte Marker (ab7779)
Anti-GFAP antibody - Astrocyte Marker (ab7779) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Protocols
References for Anti-GFAP antibody - Astrocyte Marker (ab7779)
This product has been referenced in:
- Steinman MQet al. Photoperiod interacts with food restriction in performance in the Barnes maze in female California mice. Eur J Neurosci 33:361-70 (2011). WB, IHC-Fr; Mouse.Read more (PubMed: 21198981) »
- Latimer CSet al. Reversal of glial and neurovascular markers of unhealthy brain aging by exercise in middle-aged female mice. PLoS One 6:e26812 (2011). IHC-Fr; Mouse.Read more (PubMed: 22046366) »
See all 8 publications for this product
Publishing research using ab7779? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"