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Immunohistochemistry (PFA perfusion fixed frozen sections) - GFAP antibody - Astrocyte Marker (ab7779)
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Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab7779)
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Western blot - GFAP antibody - Astrocyte Marker (ab7779)
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Product Name
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GFAP antibody - Astrocyte Marker
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See all GFAP antibodies (29)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GFAP - Astrocyte Marker
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Immunogen
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GFAP isolated from cow spinal cord.
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Reacts with
(species key)
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Hu, Ms, Rat, Cat, Cow, Dog, Shp
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Specificity
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This antibody labels the astrocytes in the brain. In peripheral neural system it reacts with Shwann cells, satellite cells, and enteric glial cells. It is positive with astrocytomas in the central neural system.
The antibody has been solid-phase absorbed with human and cow serum proteins. In crossed immunoelectrophoresis using 50 µL antibody per cm2 gel area, no reaction with 2 µL human plasma and 2 µL cow serum is observed. The antibody shows one distinct precipitate (GFAP) with cow brain extract.
In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/1000.
IHC-Fo: 1/500 - 1/1000.
IHC-P: 1/500 - 1/1000.
IHC-Fr: 1/500 - 1/1000.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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brain tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class II >> GFAP
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
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Relevance
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Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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4.500 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GFAP antibody - Astrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GFAP antibody - Astrocyte Marker images, references, products related to ab7779 and other tools.
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GFAP antibody - Astrocyte Marker images:
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 Immunohistochemistry (PFA perfusion fixed frozen sections) - GFAP antibody - Astrocyte Marker (ab7779)
ab7779 at a dilution of 1/1000, staining GFAP in astrocytes (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain tissue sections in free floating IHC (see protocol link for detailed description). Images showing astrocyte body and process: [A] 40x objective [B] 20x objective. Images coloured in Photoshop.
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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Immunocytochemistry/ Immunofluorescence - GFAP antibody - Astrocyte Marker (ab7779)
ab7779 staining human brain tissue by ICC/IF. The section was formaldehyde fixed and blocked with 3% serum (for 1 hour at 24°C) prior to incubation with ab7779 (1/1000) for 12 hours at 24°C. An Alexa Fluor® 546 conjugated goat anti-rabbit was used as the secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - GFAP antibody - Astrocyte Marker (ab7779)
GFAP antibody - Astrocyte Marker (ab7779) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 50 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
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References for GFAP antibody - Astrocyte Marker (ab7779)
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This product has been used in: (two most recent references)
Schober ME et al. Intrauterine growth restriction due to uteroplacental insufficiency decreased white matter and altered NMDAR subunit composition in juvenile rat hippocampi. Am J Physiol Regul Integr Comp Physiol 296:R681-92 (2009). PubMed: 19144756
Ali H et al. Defined serum-free culturing conditions for neural tissue engineering of human cord blood stem cells. Acta Neurobiol Exp (Wars) 69:12-23 (2009). ICC/IF; Human. PubMed: 19325637
See all 4 publication references for this product.
If you publish research using ab7779 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GFAP
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GFAP antibody - Astrocyte Marker - more information
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GFAP antibody - Astrocyte Marker - related products:
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GFAP antibody - Astrocyte Marker - other tools:
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Contact Abcam with a Technical Enquiry about GFAP antibody - Astrocyte Marker |
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