Overview

  • Product nameAnti-GFAP antibody [EP672Y]
    See all GFAP primary antibodies
  • Description
    Rabbit monoclonal [EP672Y] to GFAP
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • Positive control
    • Rat brain lysate, Human brain
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab33922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IHC-Fr 1/250 - 1/1000.
WB 1/2000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    • Tissue specificityExpressed in cells lacking fibronectin.
    • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      modifications
      Phosphorylated by PKN1.
    • Cellular localizationCytoplasm. Associated with intermediate filaments.
    • Information by UniProt
    • Database links
    • Alternative names
      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    Anti-GFAP antibody [EP672Y] images

    • ICC/IF image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    • ab33922 showing positive staining in Normal brain tissue.

    • ab33922 staining GFAP in a xenograft section, human neural stem cells in fetal mouse, by Immunohistochemistry (Frozen sections).
      Tissue was fixed in paraformaldehyde and permeabilized with 0.2% Triton X. Samples were then blocked using 3% normal donkey serum for 1.5 hours at 20°C, then incubated with ab33922 at a 1/1000 dilution for 1 hour at 20°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to Alexa Fluor 555, used at a 1/500 dilution.

      ab33922 only recognizes human astrocytes and none of the murine tissue was stained. The staining of the human GFAP fibers was always very strong, clear and reliable. Very good antibody for xenograft experiments in neuroscience!

      See Abreview

    • Anti-GFAP antibody [EP672Y] (ab33922) at 1/2000 dilution + Rat brain lysate

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab33922 showing positive staining in Astrocytoma tissue.

    • ab33922 showing negative staining in Meningioma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-GFAP antibody [EP672Y] (ab33922)

    This product has been referenced in:
    • Bility MT  et al. Hepatitis B virus infection and immunopathogenesis in a humanized mouse model: induction of human-specific liver fibrosis and M2-like macrophages. PLoS Pathog 10:e1004032 (2014). IHC-P ; Human . Read more (PubMed: 24651854) »
    • Jeerage KM  et al. Neurite outgrowth and differentiation of rat cortex progenitor cells are sensitive to lithium chloride at non-cytotoxic exposures. Neurotoxicology 33:1170-9 (2012). Read more (PubMed: 22771960) »

    See all 2 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (Differentiated hNSCs)
    Permeabilization Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy Undifferentiated Stem Cells (white)
    Specification Differentiated hNSCs
    Preparation Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (Glioma)
    Gel Running Conditions Reduced Denaturing (10% gel)
    Loading amount 25 µg
    Specification Glioma
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen retrieval: Citrate buffer 1x 3 min en olla a presiòn 150¹ (preparado a partir de 10x Heat mediated antigen retrieval solution pH:6 - 250 ml abcam ref ab973)
    Sample Mouse Tissue sections (retina with diabetic retinopathy (positive GFAP))
    Specification retina with diabetic retinopathy (positive GFAP)
    Permeabilization Yes - PBS-triton x-100 0,025%
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Mar 10 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization Yes - 0.1M PBS with 3% TritonX100
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary Astrocytes culture)
    Specification Primary Astrocytes culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human neutrophil cell lysate)
    Loading amount 100 µg
    Specification human neutrophil cell lysate
    Gel Running Conditions Reduced Denaturing (7.5% of gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Xenograft: human neural stem cells in fetal mouse)
    Specification Xenograft: human neural stem cells in fetal mouse
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2 % Triton X
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Mr. Silvan Francke

    Verified customer

    Submitted Jun 13 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"