The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/250 - 1/1000.
1/2000. Predicted molecular weight: 50 kDa.
Use at an assay dependent concentration.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Tissue specificityExpressed in cells lacking fibronectin.
Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsPhosphorylated by PKN1.
Cellular localizationCytoplasm. Associated with intermediate filaments.
Immunocytochemistry/ Immunofluorescence - Anti-GFAP antibody [EP672Y] (ab33922)Image Courtesy of Ruma Raha-Chowdhury
ICC/IF image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.
ab33922 showing positive staining in Normal brain tissue.
Immunohistochemistry (Frozen sections) - GFAP antibody [EP672Y] (ab33922)Image courtesy of Silvan Francke by Abreview.
ab33922 staining GFAP in a xenograft section, human neural stem cells in fetal mouse, by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and permeabilized with 0.2% Triton X. Samples were then blocked using 3% normal donkey serum for 1.5 hours at 20°C, then incubated with ab33922 at a 1/1000 dilution for 1 hour at 20°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to Alexa Fluor 555, used at a 1/500 dilution. ab33922 only recognizes human astrocytes and none of the murine tissue was stained. The staining of the human GFAP fibers was always very strong, clear and reliable. Very good antibody for xenograft experiments in neuroscience!
References for Anti-GFAP antibody [EP672Y] (ab33922)
This product has been referenced in:
Bility MT et al. Hepatitis B virus infection and immunopathogenesis in a humanized mouse model: induction of human-specific liver fibrosis and M2-like macrophages. PLoS Pathog10:e1004032 (2014).
Read more (PubMed: 24651854) »
Jeerage KM et al. Neurite outgrowth and differentiation of rat cortex progenitor cells are sensitive to lithium chloride at non-cytotoxic exposures. Neurotoxicology33:1170-9 (2012).
Read more (PubMed: 22771960) »