Overview

  • Product name
    Anti-GFAP antibody [EP672Y]
    See all GFAP primary antibodies
  • Description
    Rabbit monoclonal [EP672Y] to GFAP
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • Positive control
    • Rat brain lysate, Human brain
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab33922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IHC-Fr 1/250 - 1/1000.
WB 1/2000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
    • Tissue specificity
      Expressed in cells lacking fibronectin.
    • Involvement in disease
      Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Post-translational
      modifications
      Phosphorylated by PKN1.
    • Cellular localization
      Cytoplasm. Associated with intermediate filaments.
    • Information by UniProt
    • Database links
    • Alternative names
      • wu:fb34h11 antibody
      • ALXDRD antibody
      • cb345 antibody
      • etID36982.3 antibody
      • FLJ42474 antibody
      • FLJ45472 antibody
      • GFAP antibody
      • GFAP_HUMAN antibody
      • gfapl antibody
      • Glial fibrillary acidic protein antibody
      • Intermediate filament protein antibody
      • wu:fk42c12 antibody
      • xx:af506734 antibody
      • zgc:110485 antibody
      see all

    Anti-GFAP antibody [EP672Y] images

    • ICC/IF image of ab33922 stained Rat primary mixed astrocytes culture. The cells were 100% Paraformaldehyde fixed and then incubated in 10% Serum / 0.1M PBS with 10% Donkey serum for 4h. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution.

    • ab33922 showing positive staining in Normal brain tissue.

    • ab33922 staining GFAP in a xenograft section, human neural stem cells in fetal mouse, by Immunohistochemistry (Frozen sections).
      Tissue was fixed in paraformaldehyde and permeabilized with 0.2% Triton X. Samples were then blocked using 3% normal donkey serum for 1.5 hours at 20°C, then incubated with ab33922 at a 1/1000 dilution for 1 hour at 20°C. The secondary used was a donkey anti-rabbit polyclonal conjugated to Alexa Fluor 555, used at a 1/500 dilution.

      ab33922 only recognizes human astrocytes and none of the murine tissue was stained. The staining of the human GFAP fibers was always very strong, clear and reliable. Very good antibody for xenograft experiments in neuroscience!

      See Abreview

    • Anti-GFAP antibody [EP672Y] (ab33922) at 1/2000 dilution + Rat brain lysate

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab33922 showing positive staining in Astrocytoma tissue.

    • ab33922 showing negative staining in Meningioma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-GFAP antibody [EP672Y] (ab33922)

    This product has been referenced in:
    • Enkhjargal B  et al. Intranasal administration of vitamin D attenuates blood-brain barrier disruption through endogenous upregulation of osteopontin and activation of CD44/P-gp glycosylation signaling after subarachnoid hemorrhage in rats. J Cereb Blood Flow Metab N/A:271678X16671147 (2016). Rat . Read more (PubMed: 27671249) »
    • Kemp KC  et al. Cytokine therapy-mediated neuroprotection in a Friedreich's ataxia mouse model. Ann Neurol N/A:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 28009062) »

    See all 6 Publications for this product

    Product Wall

    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioma)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    25 µg
    Specification
    Glioma
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen retrieval: Citrate buffer 1x 3 min en olla a presiòn 150¹ (preparado a partir de 10x Heat mediated antigen retrieval solution pH:6 - 250 ml abcam ref ab973)
    Sample
    Mouse Tissue sections (retina with diabetic retinopathy (positive GFAP))
    Specification
    retina with diabetic retinopathy (positive GFAP)
    Permeabilization
    Yes - PBS-triton x-100 0,025%
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Mar 10 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 13 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary Astrocytes culture)
    Specification
    Primary Astrocytes culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human neutrophil cell lysate)
    Loading amount
    100 µg
    Specification
    human neutrophil cell lysate
    Gel Running Conditions
    Reduced Denaturing (7.5% of gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Xenograft: human neural stem cells in fetal mouse)
    Specification
    Xenograft: human neural stem cells in fetal mouse
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2 % Triton X
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Mr. Silvan Francke

    Verified customer

    Submitted Jun 13 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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