Anti-GFAP antibody [G-A-5] (Cy3 ®) (ab49874)


  • Product nameAnti-GFAP antibody [G-A-5] (Cy3 ®)
    See all GFAP primary antibodies
  • Description
    Mouse monoclonal [G-A-5] to GFAP (Cy3 ®)
  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm
  • SpecificityRecognizes an epitope localized on the carboxy terminal Cys II fragment of GFAP and the N-terminal part of the tail sequence of the molecule.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cat, Human, Pig, Goldfish
  • Immunogen

    Purified GFAP from pig spinal cord

  • Positive control
    • Rat cerebellum.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) conjugated to Cy3®. The Cy3®-antibody conjugate is extensively dialyzed to remove unbound Cy3®.


Associated products


Our Abpromise guarantee covers the use of ab49874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/400.
IHC-P 1/400. The epitope recognized by the antibody is partially sensitive to prolonged formalin fixation, but is resistant to alcohol-fixation followed by paraffin-embedding and to acetone-fixation of frozen sections.
ICC/IF Use at an assay dependent dilution. PubMed: 20211723


  • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificityExpressed in cells lacking fibronectin.
  • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    Phosphorylated by PKN1.
  • Cellular localizationCytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

Anti-GFAP antibody [G-A-5] (Cy3 ®) images

  • Immunocytochemistry/Immunofluorescence analysis of chicken astrocytes labelling GFAP (red) with ab49874. Nuclei stained using DAPI (blue). Cells were fixed with methanol for 15 minutes, washed in PBS and permeabilized with PBST for 10 minutes. Cells were then washed and blocked using 1% BSA in PBST for 30 minutes. Cells were incubated with the primary antibody (1:400 in blocking solution) for 1 hour at room temperature or overnight in a humid chamber in 4°C. 

References for Anti-GFAP antibody [G-A-5] (Cy3 ®) (ab49874)

This product has been referenced in:
  • Liu Y  et al. PD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients. J Neurosci 33:14231-45 (2013). Read more (PubMed: 23986257) »
  • Dotan S  et al. An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells. Neurotoxicol Teratol 32:481-8 (2010). ICC/IF ; Chicken . Read more (PubMed: 20211723) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step PBS +1% BSA +1% Ovalbumine +1% goat serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 25 2012

Thank you for contacting us. I apologize for the delay in replying to your request for a Cy3-conjugated mouse IgG1 isotype control to complement the Cy3-conjugated GFAP antibody, ab49874. Unexpectedly, I was not able to find one, in our own catalogue o...

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Thank you for your enquiry. I sincerely apologize for the error found in the datasheet. I can confirm that the species reactivity is 'Snail' and the datasheet has been updated to show the changes. Thank you for pointing this out to us. Regardin...

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