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Anti-GFAP antibody [GA-5] - Astrocyte Marker, prediluted (ab9551)

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Overview

Product name

Anti-GFAP antibody [GA-5] - Astrocyte Marker, prediluted
See all GFAP products (35) ...

Description

Mouse monoclonal [GA-5] to GFAP - Astrocyte Marker, prediluted

Specificity

This antibody reacts with human GFAP. It shows no cross-reaction with other intermediate filaments. Anti-GFAP stains astrocytes, ependymal cells and corresponding tumors.

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Rat, Chicken, Human, Pig

Immunogen

GFAP isolated from porcine spinal cord.

Positive control

Normal brain or astrocytoma

General notes

GFAP is Glial Fibrillary Acidic Protein

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, Carrier protein, pH 7.3

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

GA-5

Isotype

IgG1

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-P

     

Application notes

This antibody is ready to use.
For best results use heat mediated antigen retrieval, this can enhance staining if tissues are over-fixed.
Do not use enzymatic mediated antigen retrieval.
We recommend incubating sections with the antibody for 30-60 minutes at room temperature.

Target

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue specificity

Expressed in cells lacking fibronectin.

Involvement in disease

Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational
modifications

Phosphorylated by PKN1.

Cellular localization

Cytoplasm. Associated with intermediate filaments.

Target information above from: UniProt accessionP14136 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Astrocyte antibody
  • FLJ42474 antibody
  • FLJ45472 antibody
  • GFAP antibody
  • GFAP_HUMAN antibody
  • Glial Fibrillary Acidic Protein antibody
  • Intermediate filament protein antibody
see all

References for Anti-GFAP antibody [GA-5] - Astrocyte Marker, prediluted (ab9551)

This product has been referenced in:

  • Grace CEet al. Effect of a neurotoxic dose regimen of (+)-methamphetamine on behavior, plasma corticosterone, and brain monoamines in adult C57BL/6 mice. Neurotoxicol Teratol 32:346-55 (2010).Read more (PubMed: 20096350) »

See 1 publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"