The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
Use at an assay dependent concentration.
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Expressed in cells lacking fibronectin.
Involvement in disease
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Belongs to the intermediate filament family.
Phosphorylated by PKN1.
Cytoplasm. Associated with intermediate filaments.
Immunoprecipitation Western Blot for ab154474.
All lanes: ab154474 at 0.5 µg/ml.
Lane 1: Zebrafish Brain Homogenate lysate 10 µg
Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.
Yang Q et al. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol3:484-499 (2017).
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