Anti-GFAP (phospho S8) antibody [YC10] (ab115898)

Overview

  • Product nameAnti-GFAP (phospho S8) antibody [YC10]
    See all GFAP primary antibodies
  • Description
    Mouse monoclonal [YC10] to GFAP (phospho S8)
  • Tested applicationsSuitable for: ELISA, Functional Studies, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to the N-terminus of Pig GFAP phosphorylated at Ser8.

Properties

Applications

Our Abpromise guarantee covers the use of ab115898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Functional Studies Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
  • Tissue specificityExpressed in cells lacking fibronectin.
  • Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylated by PKN1.
  • Cellular localizationCytoplasm. Associated with intermediate filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • wu:fb34h11 antibody
    • ALXDRD antibody
    • cb345 antibody
    • etID36982.3 antibody
    • FLJ42474 antibody
    • FLJ45472 antibody
    • GFAP antibody
    • GFAP_HUMAN antibody
    • gfapl antibody
    • Glial fibrillary acidic protein antibody
    • Intermediate filament protein antibody
    • wu:fk42c12 antibody
    • xx:af506734 antibody
    • zgc:110485 antibody
    see all

References for Anti-GFAP (phospho S8) antibody [YC10] (ab115898)

This product has been referenced in:
  • Matsuoka Y  et al. Two different protein kinases act on a different time schedule as glial filament kinases during mitosis. EMBO J 11:2895-902 (1992). WB, ICC ; Human . Read more (PubMed: 1379174) »
  • Yano T  et al. A monoclonal antibody to the phosphorylated form of glial fibrillary acidic protein: application to a non-radioactive method for measuring protein kinase activities. Biochem Biophys Res Commun 175:1144-51 (1991). Read more (PubMed: 2025246) »

See all 2 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (myenteric plexus)
Specification myenteric plexus
Username

Dr. Ben Lee

Verified customer

Submitted Nov 21 2013

We are not aware of any immunohistochemistry data for this clone, YC10. I have added a reference to the datasheet from the laboratory that developed the clone in which staining of cell cultures (immunocytochemistry) is discussed, with data: Matsuoka...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"