Overview

  • Product nameAnti-GFI1 antibody
    See all GFI1 primary antibodies
  • Description
    Rabbit polyclonal to GFI1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 280-330 of Human GFI1 (Q99684).

  • Positive control
    • HepG2 and RAW cell lysates, Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab107589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 - 8 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
  • Involvement in diseaseDefects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
    Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • DomainThe Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GFI 1 antibody
    • GFI1 antibody
    • GFI1_HUMAN antibody
    • Growth factor independence 1 antibody
    • Growth factor independent 1 antibody
    • Growth factor independent protein 1 antibody
    • SCN2 antibody
    • Zinc finger protein 163 antibody
    • Zinc finger protein Gfi 1 antibody
    • Zinc finger protein Gfi-1 antibody
    • Zinc finger protein Gfi1 antibody
    • ZNF 163 antibody
    • ZNF163 antibody
    see all

Anti-GFI1 antibody images

  • All lanes : Anti-GFI1 antibody (ab107589) at 6 µg/ml

    Lane 1 : Human HepG2 cell lysate
    Lane 2 : Human HepG2 cell lysate with Immunizing peptide
    Lane 3 : Mouse RAW cell lysate

    Secondary
    Goat anti-rabbit HRP
    Developed using the ECL technique

    Predicted band size : 45 kDa
  • Immunohistochemical analysis of GFI1 in formalin-fixed, paraffin-embedded human brain tissue using ab107589 at 5 µg/ml.

References for Anti-GFI1 antibody (ab107589)

ab107589 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (Cochlea)
Specification Cochlea
Username

Ms. Michelle Randle

Verified customer

Submitted Oct 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"