Overview

  • Product name
    Anti-GFI1 antibody
  • Description
    Rabbit polyclonal to GFI1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human GFI1.

    (Peptide available as ab22461.)

  • Positive control
    • THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)

Properties

Applications

Our Abpromise guarantee covers the use of ab21061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).Can be blocked with GFI1 peptide (ab222418).

Target

  • Function
    Transcription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
  • Involvement in disease
    Defects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
    Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
  • Sequence similarities
    Contains 6 C2H2-type zinc fingers.
  • Domain
    The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GFI 1 antibody
    • GFI1 antibody
    • GFI1_HUMAN antibody
    • Growth factor independence 1 antibody
    • Growth factor independent 1 antibody
    • Growth factor independent protein 1 antibody
    • SCN2 antibody
    • Zinc finger protein 163 antibody
    • Zinc finger protein Gfi 1 antibody
    • Zinc finger protein Gfi-1 antibody
    • Zinc finger protein Gfi1 antibody
    • ZNF 163 antibody
    • ZNF163 antibody
    see all

Images

  • All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/ml

    Lane 1 : THP1 whole cell lysate (ab7913)
    Lane 2 : THP1 whole cell lysate (ab7913) with Human GFI1 peptide (ab22461) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680)

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa (possible cleavage fragment,cross reactivity).

    ab21061 detects a band of ~49kDa which is similar to the expected size of GFI1 (45kDa). This band is competed away by incubation with the immunizing peptide. A smaller band (~36kDa) is seen that may correspond to a crossreacting peptide or to a degradation product, as it is also competed away by addition of the immunizing peptide.

References

This product has been referenced in:
  • Collombet S  et al. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc Natl Acad Sci U S A 114:5792-5799 (2017). WB . Read more (PubMed: 28584084) »
  • Moignard V  et al. Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis. Nat Cell Biol 15:363-72 (2013). Read more (PubMed: 23524953) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Axolotl limb 30 days post amputation)
Permeabilization
Yes - Dako
Specification
Axolotl limb 30 days post amputation
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C
Fixative
Acetone
Username

Crystal Jones

Verified customer

Submitted Nov 17 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (Hematopoietic progenitors)
Specification
Hematopoietic progenitors
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Paraformaldehyde
Detection step
Real-time PCR
Positive control
GFI-1 locus
Negative control
Alpha actin locus
Username

Abcam user community

Verified customer

Submitted Jun 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL-60, Jurkat, Raw264.7, PC12)
Loading amount
20 µg
Specification
HL-60, Jurkat, Raw264.7, PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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