Overview

  • Product nameAnti-GFI1 antibody
    See all GFI1 primary antibodies
  • Description
    Mouse monoclonal to GFI1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ LTEAPDRASA SPRQLRSSVC ERSSEFEDFW R, corresponding to amino acids 1-92 of Human GFI1

Properties

Applications

Our Abpromise guarantee covers the use of ab55108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionTranscription factor regulating the expression of genes active during S-phase of the cell cycle in hematopoietic cells. Represses ELA2 transcription.
  • Involvement in diseaseDefects in GFI1 are a cause of neutropenia severe congenital autosomal dominant type 2 (SCN2) [MIM:613107]. SCN2 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
    Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • DomainThe Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GFI 1 antibody
    • GFI1 antibody
    • GFI1_HUMAN antibody
    • Growth factor independence 1 antibody
    • Growth factor independent 1 antibody
    • Growth factor independent protein 1 antibody
    • SCN2 antibody
    • Zinc finger protein 163 antibody
    • Zinc finger protein Gfi 1 antibody
    • Zinc finger protein Gfi-1 antibody
    • Zinc finger protein Gfi1 antibody
    • ZNF 163 antibody
    • ZNF163 antibody
    see all

Anti-GFI1 antibody images

  • GFI1 antibody (ab55108) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
  • Western blot against tagged recombinant protein immunogen using ab55108 GFI1 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • ab55108 at 10 ug/ml staining GFl1 in human Hela cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-GFI1 antibody (ab55108)

ab55108 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"