Overview

  • Product nameAnti-GFI1B antibody
    See all GFI1B primary antibodies
  • Description
    Rabbit polyclonal to GFI1B
  • Specificityab26132 recognises GFI1B protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide derived from a region between residues 1 - 50 of human GFI1B.

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  • Positive control
    • Jurkat cell lysate, human heart tissue, human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab26132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionEssential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
  • Tissue specificityExpressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA).
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • DomainThe zinc finger domains are essential for erythroid expansion and acts as an activation domain whereas non finger domain serves as repression domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GFI 1B antibody
    • gfi1b antibody
    • GFI1B protein antibody
    • GFI1B_HUMAN antibody
    • Growth factor independent 1B protein antibody
    • Growth factor independent 1B transcription repressor antibody
    • Growth factor independent protein 1B antibody
    • OTTHUMP00000022443 antibody
    • OTTHUMP00000022444 antibody
    • OTTHUMP000000235527 antibody
    • Potential regulator of CDKN1A antibody
    • Potential regulator of CDKN1A translocated in CML antibody
    • Translocated in CML antibody
    • Zinc finger protein Gfi-1b antibody
    see all

Anti-GFI1B antibody images

  • Anti-GFI1B antibody (ab26132) at 1 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Paraffin-embedded sections) analysis of human heart tissue labelling GFI1B with ab26132 at 4-8µg/ml. Magnification: 400X.

  • Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling GFI1B with ab26132 at 4-8µg/ml. Magnification: 400X.

References for Anti-GFI1B antibody (ab26132)

This product has been referenced in:
  • Pinello L  et al. Analysis of chromatin-state plasticity identifies cell-type-specific regulators of H3K27me3 patterns. Proc Natl Acad Sci U S A 111:E344-53 (2014). WB, ChIP ; Human . Read more (PubMed: 24395799) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"