Overview

  • Product nameAnti-GFM1 antibody
    See all GFM1 primary antibodies
  • Description
    Rabbit polyclonal to GFM1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide between C terminal amino acids 727-755 of Human GFM1 (NP_079272.4).

  • Positive control
    • WB: NCI-H460 cell line lysates; Mouse heart tissue lysates IHC-P: Human bladder carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab107496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 83 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
  • PathwayProtein biosynthesis; polypeptide chain elongation.
  • Involvement in diseaseDefects in GFM1 are the cause of combined oxidative phosphorylation deficiency type 1 (COXPD1) [MIM:609060]. It leads to early fatal progressive hepatoencephalopathy.
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • COXPD1 antibody
    • EF-Gmt antibody
    • EFG antibody
    • EFG1 antibody
    • EFGM antibody
    • EFGM_HUMAN antibody
    • EGF1 antibody
    • Elongation factor G 1 antibody
    • Elongation factor G 1 mitochondrial antibody
    • Elongation factor G antibody
    • Elongation factor G1 antibody
    • FLJ12662 antibody
    • FLJ13632 antibody
    • FLJ20773 antibody
    • G elongation factor mitochondrial 1 antibody
    • G translation elongation factor mitochondrial antibody
    • GFM 1 antibody
    • GFM antibody
    • gfm1 antibody
    • hEFG1 antibody
    • mEF G 1 antibody
    • mEF-G 1 antibody
    • mEFG 1 antibody
    • mitochondrial antibody
    • Mitochondrial elongation factor G1 antibody
    see all

Anti-GFM1 antibody images

  • Anti-GFM1 antibody (ab107496) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 83 kDa
  • Anti-GFM1 antibody (ab107496) at 1/100 dilution + Mouse heart tissue lysates at 35 µg

    Predicted band size : 83 kDa
  • ab107496, at a 1/50 dilution, staining GFM1 in formalin fixed and paraffin embedded Human bladder carcinoma, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-GFM1 antibody (ab107496)

ab107496 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"