Overview

  • Product nameAnti-GFM2 antibody
    See all GFM2 primary antibodies
  • Description
    Rabbit polyclonal to GFM2
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human GFM2.

  • Positive control
    • Extracts from LOVO cells and RAW264.7 cells. A549 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • RelevanceGFM2 is a mitochondrial translation elongation factor. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • EF-G2mt antibody
    • EFG2 antibody
    • EFG2mt antibody
    • elongation factor G 2, mitochondrial antibody
    • Elongation factor G2 antibody
    • G elongation factor mitochondrial 2 antibody
    • GFM2 antibody
    • hEFG2 antibody
    • mEFG 2 antibody
    • mEFG2 antibody
    • Mitochondrial elongation factor G2 antibody
    • mitochondrial ribosome recycling factor 2 antibody
    • MRRF2 antibody
    • MST027 antibody
    • MSTP027 antibody
    • OTTHUMP00000222951 antibody
    • OTTHUMP00000222952 antibody
    • ribosome-releasing factor 2, mitochondrial antibody
    • RRF2 antibody
    • RRF2mt antibody
    see all

Anti-GFM2 antibody images

  • All lanes : Anti-GFM2 antibody (ab74874) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from RAW264.7 cells
    Lane 3 : extracts from LOVO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 87 kDa
    Observed band size : 87 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab74874, at a dilution of 1/500, staining GFM2 in A549 cells by Immunofluorescence in the absence (left image) or presence (right image) of the immunizing peptide.

References for Anti-GFM2 antibody (ab74874)

ab74874 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"