Overview

  • Product name
  • Description
    Chicken polyclonal to GFP
  • Specificity
    detects GFP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Immunogen

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein L purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab101863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 27 kDa.
IHC-P 1/500.

Target

  • Relevance
    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody

Anti-GFP antibody images

  • Anti-GFP antibody (ab101863) at 1/5000 dilution + transgenic mice expressing the GFP gene product with IgY blocking reagent

    Secondary
    HRP-labeled goat anti-chicken antibodies

    Predicted band size : 27 kDa
  • ab101863, at a 1/500 dilution, staining GFP in transgenic mice expressing the GFP gene product by Immunohistochemistry.

References for Anti-GFP antibody (ab101863)

This product has been referenced in:
  • Zeng X & Hou SX Enteroendocrine cells are generated from stem cells through a distinct progenitor in the adult Drosophila posterior midgut. Development 142:644-53 (2015). Read more (PubMed: 25670791) »

See 1 Publication for this product

Product Wall

At Abcam, providing quality service is just as important as providing quality products. Lack of references does not mean the antibody has not been tested. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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