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Fusion protein corresponding to Aequorea victoria GFP aa 1-246.
Database link: P42212
Our Abpromise guarantee covers the use of ab6673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/2000.
(for immunoprecipitated GFP, see Abreview).
|IP||Use at an assay dependent concentration.|
This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.
|IHC-P||1/200 - 1/1000.|
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/1000.|
|IHC - Wholemount||Use at an assay dependent concentration.|
Immunofluorescence of TGN mouse liver labeling GFP on hepatocytes with ab6673.
Immunofluorescence of E5.5 Hex-GFP transgenic mouse embryo labeling GFP with ab6673 at 1/500. Secondary antibody: Fluorchrome conjugated anti-goat IgG (1/10000) for 45 mins at room temperature. Staining GFP as green fluorescent signal with DAPI blue counterstain.
Immunohistochemistry of GFP transgenic mouse liver labeling GFP with ab6673.