For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Fusion protein corresponding to Aequorea victoria GFP aa 1-246.
Database link: P42212
Our Abpromise guarantee covers the use of ab6673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/2000.
(for immunoprecipitated GFP, see Abreview).
|IP||Use at an assay dependent concentration.|
This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.
|IHC-P||1/200 - 1/1000.|
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/1000.|
|IHC - Wholemount||Use at an assay dependent concentration.|
Immunocytochemistry/ Immunofluorescence of TGN mouse liver labeling GFP on hepatocytes with ab6673.
Immunocytochemistry/ Immunofluorescence of E5.5 Hex-GFP transgenic mouse embryo labeling GFP with ab6673 at 1/500. Secondary antibody: Fluorchrome conjugated anti-goat IgG (1/10000) for 45 mins at room temperature. Staining GFP as green fluorescent signal with DAPI blue counterstain.
This image is courtesy of an anonymous abreview.
Immunohistochemistry of GFP transgenic mouse liver labeling GFP with ab6673.