Anti-GFP antibody (ab92456)
Key features and details
- Chicken polyclonal to GFP
- Suitable for: ELISA, ICC, WB
- Reacts with: Species independent
- Isotype: IgY
Overview
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Product name
Anti-GFP antibody
See all GFP primary antibodies -
Description
Chicken polyclonal to GFP -
Host species
Chicken -
Tested applications
Suitable for: ELISA, ICC, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to Aequorea victoria GFP.
Database link: P42212 -
Positive control
- Green Fluorescence Protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/500 - 1/2000.
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ICC |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 27 kDa.
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Notes |
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ELISA
1/500 - 1/2000. |
ICC
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 27 kDa. |
Target
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Relevance
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab92456 has been referenced in 6 publications.
- Dairaghi L et al. The Dopamine D4 Receptor Regulates Gonadotropin-Releasing Hormone Neuron Excitability in Male Mice. eNeuro 9:N/A (2022). PubMed: 35165199
- Hertenstein H et al. Starvation-induced regulation of carbohydrate transport at the blood-brain barrier is TGF-ß-signaling dependent. Elife 10:N/A (2021). PubMed: 34032568
- Cheriyamkunnel SJ et al. A neuronal mechanism controlling the choice between feeding and sexual behaviors in Drosophila. Curr Biol 31:4231-4245.e4 (2021). PubMed: 34358444
- Toft CJ et al. Delineation of the Ancestral Tus-Dependent Replication Fork Trap. Int J Mol Sci 22:N/A (2021). PubMed: 34948327
- Trébuchet G et al. The Repo Homeodomain Transcription Factor Suppresses Hematopoiesis in Drosophila and Preserves the Glial Fate. J Neurosci 39:238-255 (2019). PubMed: 30504274
- Lee JK et al. Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length. Elife 6:N/A (2017). PubMed: 29231808