Anti-GFP antibody (Alkaline Phosphatase) (ab6661)


  • Product nameAnti-GFP antibody (Alkaline Phosphatase)
    See all GFP primary antibodies
  • Description
    Goat polyclonal to GFP (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • SpecificityAntibody recognizes wild type, recombinant and enhanced forms of GFP. Immunoblot shows a 42 kDa band when reacted with GFP on a Western blot. No reaction was observed against human, mouse and rat serum proteins.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

  • General notes

    Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 50mM Tris HCl, 150mM Sodium chloride, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, anti-Alkaline Phosphatase and purified and partially purified GFP (Aequorea victoria) serum.
  • Primary antibody notesDesigned to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500.
ELISA 1/2000 - 1/8000. This product has been assayed against 1.0 microg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.


  • RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Anti-GFP antibody (Alkaline Phosphatase) images

  • Anti-GFP antibody (Alkaline Phosphatase) (ab6661) at 1/1000 dilution + GFP at 0.05 µg with Blocking buffer
  • Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.


    Lane 1: 100 ng GFP protein
    Lane 2: 33.33 ng GFP protein
    Lane 3: 11.11 ng GFP protein
    Lane 4: 3.70 ng GFP protein
    Lane 5: 1.23 ng GFP protein

References for Anti-GFP antibody (Alkaline Phosphatase) (ab6661)

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Product Wall

Thank you for your enquiry. This antibody is suitable for use in Western blotting. It shows a 42 kDa band when reacted with GFP on a Western blot, and the antibody recognizes wild type, recombinant and enhanced forms of GFP. If you have any additio...

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The ELISA data is using 1ug to coat the wells and is used as a control not as a minimum level of detection. Unfortunately we have no data on the detection limit in ELISA.

To our knowledge this product has yet to be tested in immunihistochemistry. We are very keen for information regarding this application.

You can use any standard sandwich ELISA protocol. Use ab6673 (goat anti-GFP) as capturing antibody at 1 microg/well and ab6661 at a dilution of 1:1,000 to 1:5,000.