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The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500.|
|ELISA||1/2000 - 1/8000. This product has been assayed against 1.0 microg of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.|
Dot blott analysis of ab6661 used in all lanes at a concentration of 1/1,000 incubated at room temperature for 1 hour.
Lane 1: 100 ng GFP protein
Lane 2: 33.33 ng GFP protein
Lane 3: 11.11 ng GFP protein
Lane 4: 3.70 ng GFP protein
Lane 5: 1.23 ng GFP protein