Recombinant
RabMAb

Anti-GFP antibody [E385] (ab32146)

Overview

  • Product name
    Anti-GFP antibody [E385]
    See all GFP primary antibodies
  • Description
    Rabbit monoclonal [E385] to GFP
  • Specificity
    This antibody is specific for GFP and GFP fusion proteins.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IP
  • Immunogen

    within Aequorea victoria GFP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P42212

  • General notes

    On the basis of low sequence homology, ab32146 is predicted to show no or limited cross-reactivity to RFP, YFP, BFP, and CFP

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)
    KD = 1.02 x 10 -12 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    E385
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000.

GFP - 27kDa, Proprietary tag/GFP fusion protein - 52kDa

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance
      Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

      Subunit structure: Monomer.

      Tissue specificity: Photocytes.

      Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      Sequence similarities: Belongs to the GFP family.

      Biophysicochemical properties: Absorption: Abs(max)=395 nm
      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
    • Alternative names
      • GFP antibody
      • Green fluorescent protein antibody

    Images

    • All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (purified)

      Lane 1 : HeLa whole cell lysate (negative control)
      Lane 2 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg
      Lane 3 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg
      Lane 4 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (unpurified)

      Lane 1 : HeLa cells lysate (negative control)
      Lane 2 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng
      Lane 3 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng
      Lane 4 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng
      Lane 5 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng
      Lane 6 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng
      Lane 7 : Proprietary tag-GFP 0.1mg/ml (positive control)

    References

    This product has been referenced in:

    See all 16 Publications for this product

    Customer reviews and Q&As

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A
    Sample
    Mouse Cell lysate - whole cell (NIH3T3 fibroblast)
    Specification
    NIH3T3 fibroblast
    Total protein in input
    500 µg
    Username

    Dr. Tai yu Lai

    Verified customer

    Submitted Apr 25 2014

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293 cells)
    Specification
    HEK293 cells
    Treatment
    transfected with GFP-only expression plasmid for 48h
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 04 2013

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 19 2013

    >Der ab32146 Anti-GFP antibody [E385]sollte für Ihre Zwecke geeignet sein, da dessen Immunogen in den ersten 30 AS zu finden ist.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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