Overview

  • Product nameAnti-GFP antibody [E385]
    See all GFP primary antibodies
  • Description
    Rabbit monoclonal [E385] to GFP
  • SpecificityThis antibody is specific for GFP and GFP fusion proteins.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IP
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Aequorea victoria GFP aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    On the basis of low sequence homology, ab32146 is predicted to show no or limited cross-reactivity to RFP, YFP, BFP, and CFP

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-GFP antibody (Alexa Fluor® 488) [E385] (ab190203)

    Anti-GFP antibody (Alexa Fluor® 647) [E385] (ab190585)

    Anti-GFP antibody (Alexa Fluor® 555) [E385] (ab203424)

    Anti-GFP antibody (HRP) [E385] (ab190584)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD) KD = 1.02 x 10 -12 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberE385
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000.

GFP - 27kDa, Proprietary tag/GFP fusion protein - 52kDa

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

      Subunit structure: Monomer.

      Tissue specificity: Photocytes.

      Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      Sequence similarities: Belongs to the GFP family.

      Biophysicochemical properties: Absorption: Abs(max)=395 nm
      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
    • Alternative names
      • GFP antibody
      • Green fluorescent protein antibody
      • yfp antibody

    Anti-GFP antibody [E385] images

    • All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (purified)

      Lane 1 : HeLa whole cell lysate (negative control)
      Lane 2 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg
      Lane 3 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg
      Lane 4 : HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution (unpurified)

      Lane 1 : HeLa cells lysate (negative control)
      Lane 2 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng
      Lane 3 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng
      Lane 4 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng
      Lane 5 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng
      Lane 6 : HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng
      Lane 7 : Proprietary tag-GFP 0.1mg/ml (positive control)

    References for Anti-GFP antibody [E385] (ab32146)

    This product has been referenced in:
    • Chen D  et al. Human herpesvirus 8 interleukin-6 contributes to primary effusion lymphoma cell viability via suppression of proapoptotic cathepsin D, a cointeraction partner of vitamin K epoxide reductase complex subunit 1 variant 2. J Virol 88:1025-38 (2014). Read more (PubMed: 24198402) »
    • Hochbaum DR  et al. All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Nat Methods 11:825-33 (2014). Read more (PubMed: 24952910) »

    See all 11 Publications for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein A
    Sample Mouse Cell lysate - whole cell (NIH3T3 fibroblast)
    Specification NIH3T3 fibroblast
    Total protein in input 500 µg
    Username

    Dr. Tai yu Lai

    Verified customer

    Submitted Apr 25 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Specification HEK293 cells
    Treatment transfected with GFP-only expression plasmid for 48h
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 04 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 19 2013

    >Der ab32146 Anti-GFP antibody [E385]sollte für Ihre Zwecke geeignet sein, da dessen Immunogen in den ersten 30 AS zu finden ist.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"