• Product nameAnti-GFP antibody (FITC)
    See all GFP primary antibodies
  • Description
    Rabbit polyclonal to GFP (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis product is affinity purified antibody (ab6556) covalently linked to FITC. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Not applicable.
  • Immunogen

    Highly purified recombinant full length protein made in Escherichia coli. The antibody is directed against the entire GFP molecule.

  • General notesThis product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab66180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.

    Unconjugated version: ab6556.
    HRP conjugated version: ab69312.
    Biotin conjugated version: ab69313.
    Sepharose beads version: ab69314.
    Magnetic beads version: ab69315.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

      Subunit structure: Monomer.

      Tissue specificity: Photocytes.

      Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      Sequence similarities: Belongs to the GFP family.

      Biophysicochemical properties: Absorption: Abs(max)=395 nm
      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
    • Alternative names
      • GFP antibody
      • Green fluorescent protein antibody
      • yfp antibody

    Anti-GFP antibody (FITC) images

    • Following fixation with 1X PBS, 3.5 % formaldehyde, pCMV5-Yes-GFP transfected COS-7 cells were permeabilized with 1X PBS, 0.1 % Triton-X100 (A and B) as described in (McCabe and Berthiaume 1999) and GFP fluorescence was quenched by treatment with 100 mM MES pH 6.5 for 30 minutes (C) (Berthiaume, L.G., unpublished). Cells were then labelled with or without rabbit anti-GFP-FITC at a concentration of 1 ug/ml as described in (McCabe and Berthiaume 1999). A) 200X magnification of COS-7 cells expressing Yes-GFP, B) 1000X magnification of boxed area in A and C) 200X magnification of quenched COS-7 cells expressing Yes-GFP. 100 um bar in A and C; 20?um bar in inset B.

      McCabe, J.B. and Berthiaume, L.G. (1999) Functional roles for fatty acylated N-terminal domains in subcellular localization. Mol. Biol. Cell, 10, 3771-3786.

    References for Anti-GFP antibody (FITC) (ab66180)

    This product has been referenced in:
    • Gomes SA  et al. S-nitrosoglutathione reductase (GSNOR) enhances vasculogenesis by mesenchymal stem cells. Proc Natl Acad Sci U S A 110:2834-9 (2013). ICC/IF . Read more (PubMed: 23288904) »

    See 1 Publication for this product

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