Overview

  • Product name
    Anti-GFP antibody (FITC)
    See all GFP primary antibodies
  • Description
    Rabbit polyclonal to GFP (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This product is affinity purified antibody (ab6556) covalently linked to FITC. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Not applicable.
  • Immunogen

    Recombinant full length protein corresponding to GFP.
    Database link: P42212

  • General notes
    This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.

    Unconjugated version: ab6556.
    HRP conjugated version: ab69312.
    Biotin conjugated version: ab69313.
    Sepharose beads version: ab69314.
    Magnetic beads version: ab69315.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

      Subunit structure: Monomer.

      Tissue specificity: Photocytes.

      Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

      Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

      Sequence similarities: Belongs to the GFP family.

      Biophysicochemical properties: Absorption: Abs(max)=395 nm
      Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
    • Alternative names
      • GFP antibody
      • Green fluorescent protein antibody

    Images

    • Following fixation with 1X PBS, 3.5 % formaldehyde, pCMV5-Yes-GFP transfected COS-7 cells were permeabilized with 1X PBS, 0.1 % Triton-X100 (A and B) as described in (McCabe and Berthiaume 1999) and GFP fluorescence was quenched by treatment with 100 mM MES pH 6.5 for 30 minutes (C) (Berthiaume, L.G., unpublished). Cells were then labelled with or without rabbit anti-GFP-FITC at a concentration of 1 ug/ml as described in (McCabe and Berthiaume 1999). A) 200X magnification of COS-7 cells expressing Yes-GFP, B) 1000X magnification of boxed area in A and C) 200X magnification of quenched COS-7 cells expressing Yes-GFP. 100 um bar in A and C; 20?um bar in inset B.

      References
      McCabe, J.B. and Berthiaume, L.G. (1999) Functional roles for fatty acylated N-terminal domains in subcellular localization. Mol. Biol. Cell, 10, 3771-3786.

    References

    This product has been referenced in:
    • Mokhtari S  et al. Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation. Stem Cell Reports 6:957-69 (2016). Read more (PubMed: 27304918) »
    • Gomes SA  et al. S-nitrosoglutathione reductase (GSNOR) enhances vasculogenesis by mesenchymal stem cells. Proc Natl Acad Sci U S A 110:2834-9 (2013). ICC/IF . Read more (PubMed: 23288904) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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