Overview

  • Product nameAnti-GFP antibody [LGB-1] (FITC)
    See all GFP primary antibodies
  • Description
    Mouse monoclonal [LGB-1] to GFP (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody recognizes all forms of GFP from Aquorea victoria (i.e. GFP, EGFP, YFP and CFP).
  • Tested applicationsSuitable for: ICC/IFmore details
  • Immunogen

    Highly purified recombinant full length GFP expressed in Escherichia coli

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberLGB-1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceFunction: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Anti-GFP antibody [LGB-1] (FITC) images

  • ab42560 at 1/200 staining C57BL/6 mouse macrophages infected with Plasmodium berghei GFP-expressing sporozoites by ICC/IF.

    The cells were formaldehyde fixed and saponin permeabilized before incubation with ab42560 for 1 hr at 20°C.   Blocking agent was 1% BSA for 1.5 hr at 20°C. 

    In the image:
    Green: GFP
    Red: anti-LAMP-1
    Blue: DAPI

    See Abreview

References for Anti-GFP antibody [LGB-1] (FITC) (ab42560)

This product has been referenced in:
  • Brandt N  et al. B56{beta}, a regulatory subunit of protein phosphatase 2A, interacts with CALEB/NGC and inhibits CALEB/NGC-mediated dendritic branching. FASEB J : (2008). Read more (PubMed: 18385213) »

See 1 Publication for this product

Product Wall

We have not tested this antibody in IHC, however I would recommend starting at a 1:100 to test in this application.

I am sorry to confirm that they are all raised against full length protein, and the epitopes have regrettably not been mapped. We would therefore not be able to guarantee these will be detecting the C terminus of the GFP protein.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Plasmodium berghei GFP-expressing sporozoite)
Specification Plasmodium berghei GFP-expressing sporozoite
Fixative Formaldehyde
Permeabilization Yes - saponin
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Nov 12 2007

The way FITC is conjugated to this monoclonal antibody and from spectral analysis, there should be a minimum of 5 -10 molecules of FITC attached per molecules of IgG. The estimated non-conjugated antibody should be very low but have not been tested/con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"