Recombinant

Anti-GFP VHH Single Domain antibody (Magnetic beads) (ab193983)

Overview

  • Product name
    Anti-GFP VHH Single Domain antibody (Magnetic beads)
    See all GFP VHH Single Domain primary antibodies
  • Conjugation
    Magnetic beads
  • Tested applications
    Suitable for: IPmore details
  • Immunogen

    Recombinant full length protein. ab193983 is a VHH single domain antibody targeting GFP

  • Positive control
    • IP: HEK293 whole cell lysate - Overexpressing GFP, Recombinant GFP protein and GFP Fusion protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Do Not Freeze.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, Sodium phosphate, Sodium chloride
  • Concentration information loading...
  • Purity
    Purified via His tag
  • Clonality
    Monoclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Target

  • Relevance
    Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Absorption: Abs(max)=395 nm. Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Images

  • Lane 1: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul recombinant GFP (ab84191)

    Lane 2: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul GFP fusion protein.

    Lane 3: Off-target sdAb beads: Anti-Rabbit VHH Single Domain Antibody (Magnetic) + 10ul recombinant GFP (ab84191)

    Lane 4: Anti-GFP antibody (ab290) + Protein A agarose + 10ul recombinant GFP (ab84191)

    Lane 5: Anti-GFP antibody (ab290) + Protein A agarose + 10ul fusion GFP

     

    Anti-GFP antibody (Magnetic beads) (ab193983) (20ul slurry) was used to pull down recombinant GFP (ab84191) and GFP fusion protein. ab290 (Rabbit polyclonal to GFP - ChIP Grade) and Protein A agarose was used as a positive control. The gel was stained with Optiblot Blue (ab119211).

  • Lane 1: HEK293 cell lysate overexpressing GFP - 10ug

     Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 5: HEK293 cell lysate overexpressing GFP - 10ug

    Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP

    Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP

     

    Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-GFP (ab1218) and anti-mouse HRP (ab97040).

  • Lane 1: HEK293 cell lysate overexpressing GFP - 10ug

    Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

    Lane 5: HEK293 cell lysate overexpressing GFP - 10ug

    Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP

    Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP

     

    Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was stained with Optiblot Blue (ab119211).

References

This product has been referenced in:
  • Lee S  et al. Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis. Neuron 91:41-55 (2016). IP . Read more (PubMed: 27321923) »

See 1 Publication for this product

Customer reviews and Q&As

This has been tested, and it does cross react with eGFP.

After checking with the lab I can confirm you that 20 ul of magnetic bead conjugated antibody was used for the IP experiments present on the datasheet. Hence I would recommend you to start with this amount in your experiment.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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