Recombinant
RabMAb

Anti-GFPT1 antibody [EPR4854] (ab125069)

Overview

  • Product name
    Anti-GFPT1 antibody [EPR4854]
    See all GFPT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4854] to GFPT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GFPT1 aa 600-700. The exact sequence is proprietary.

  • Positive control
    • Human testis tissue; Human placenta lysate, 293T, JAR and HeLa cell line lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
    • Tissue specificity
      Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.
    • Pathway
      Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.
    • Involvement in disease
      Defects in GFPT1 are the cause of limb-girdle myasthenia with tubular aggregates (LGMTA) [MIM:610542]. A congenital myasthenic syndrome characterized by onset of proximal muscle weakness in the first decade. Individuals with this condition have a recognizable pattern of weakness of shoulder and pelvic girdle muscles, and sparing of ocular or facial muscles. EMG classically shows a decremental response to repeated nerve stimulation, a sign of neuromuscular junction dysfunction. Affected individuals show a favorable response to acetylcholinesterase (AChE) inhibitors.
    • Sequence similarities
      Contains 1 glutamine amidotransferase type-2 domain.
      Contains 2 SIS domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMS12 antibody
      • CMSTA1 antibody
      • D-fructose-6-phosphate amidotransferase 1 antibody
      • GFA antibody
      • GFAT 1 antibody
      • GFAT antibody
      • GFAT1 antibody
      • GFAT1m antibody
      • GFPT antibody
      • Gfpt1 antibody
      • GFPT1_HUMAN antibody
      • GFPT1L antibody
      • Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 antibody
      • Glutamine--fructose-6-phosphate transaminase 1 antibody
      • Glutamine:fructose 6 phosphate amidotransferase 1 antibody
      • Hexosephosphate aminotransferase 1 antibody
      • MSLG antibody
      see all

    Images

    • ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.
    • All lanes : Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 79 kDa

    References

    This product has been referenced in:
    • Shi H  et al. Skeletal muscle O-GlcNAc transferase is important for muscle energy homeostasis and whole-body insulin sensitivity. Mol Metab N/A:N/A (2018). Read more (PubMed: 29525407) »
    • Kavanagh Williamson M  et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 12 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HBEC cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Treatment
    glucose deprivation
    Specification
    HBEC cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up