Overview

  • Product nameAnti-GFPT2 antibody
    See all GFPT2 primary antibodies
  • Description
    Mouse polyclonal to GFPT2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GFPT2 (AAH00012.1, 1 a.a. ~ 682 a.a) full-length human protein.

  • Positive control
    • GFPT2 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).

Target

  • FunctionControls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.
  • Tissue specificityHighest levels of expression in heart, placenta, and spinal cord.
  • PathwayNucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.
  • Sequence similaritiesContains 1 glutamine amidotransferase type-2 domain.
    Contains 2 SIS domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • D fructose 6 phosphate amidotransferase 2 antibody
    • D-fructose-6-phosphate amidotransferase 2 antibody
    • GFAT 2 antibody
    • GFAT2 antibody
    • Gfpt2 antibody
    • GFPT2_HUMAN antibody
    • Glucosamine fructose 6 phosphate aminotransferase [isomerizing] 2 antibody
    • glutamine fructose 6 phosphate transaminase 2 antibody
    • Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 antibody
    • Glutamine:fructose 6 phosphate amidotransferase 2 antibody
    • Glutamine:fructose-6-phosphate amidotransferase 2 antibody
    • Hexosephosphate aminotransferase 2 antibody
    see all

Anti-GFPT2 antibody images

  • All lanes : Anti-GFPT2 antibody (ab70078) at 1/500 dilution

    Lane 1 : GFPT2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 77 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab70078 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70078, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GFPT2 antibody (ab70078)

ab70078 has not yet been referenced specifically in any publications.

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