Overview

  • Product name
  • Description
    Rabbit polyclonal to GFR alpha 3
  • Tested applications
    Suitable for: IHC (PFA fixed), IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 347-360 of Mouse GFR alpha 3.

  • Positive control
    • Murine kidney, liver or heart tissue lysates.
  • General notes


    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Members of the glial cell line-derived neurotrophic factor(GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb. Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3. GFRa-3 binds persephin, thus, persephin, GFRa-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) 1/50.
IHC-P Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.Can be blocked with GFR alpha 3 peptide (ab6100).

Target

  • Function
    Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.
  • Tissue specificity
    Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
  • Sequence similarities
    Belongs to the GDNFR family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDNF family receptor alpha 3 antibody
    • GDNF family receptor alpha-3 antibody
    • GDNF receptor alpha-3 antibody
    • GDNFR-alpha-3 antibody
    • GDNFR3 antibody
    • GFR-alpha-3 antibody
    • GFRA3 antibody
    • GFRA3_HUMAN antibody
    • Glial cell line derived neurotrophic factor receptor alpha 3 antibody
    • GPI linked receptor antibody
    • OTTHUMP00000159486 antibody
    • OTTHUMP00000224020 antibody
    see all

Anti-GFR alpha 3 antibody images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling GFR alpha 3 with Anti-GFR alpha 3 antibody (ab8028) at 5μg/ml.
  • All lanes : Anti-GFR alpha 3 antibody (ab8028) at 1/500 dilution

    Lane 1 : Crude cell membrane fractions of murine heart
    Lane 2 : Crude cell membrane fractions of murine spleen
    Lane 3 : Crude cell membrane fractions of murine kidney
    Lane 4 : Crude cell membrane fractions of murine liver
    Lane 5 : Crude cell membrane fractions of murine brain


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Western blot analysis of GFR alpha 3 in crude cell membrane fractions of
    murine heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with ab8028 at 1/500 dilution.
  • ab8028 at 10µg/ml staining GFR alpha 3 in mouse heart tissue by IHC-P.

  • Immunohistochemical analysis of cleavage-stage Human embryo, staining GFR alpha 3 (green) with ab8028.

    Embyros were fixed with 4% paraformaldehyde for 30 min at 23°C. After permeabilization with 0.1% Triton X-100, embryos were pre-incubated in 5% BSA for 1 hour before incubation with primary antibody (diluted in PBS with 1% BSA) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.

References for Anti-GFR alpha 3 antibody (ab8028)

This product has been referenced in:
  • Kawamura K  et al. Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One 7:e49328 (2012). IHC - Wholemount ; Human . Read more (PubMed: 23152897) »
  • Ceyhan GO  et al. The neurotrophic factor artemin promotes pancreatic cancer invasion. Ann Surg 244:274-81 (2006). WB, IHC-P ; Human . Read more (PubMed: 16858191) »

See all 3 Publications for this product

Product Wall

Application
Flow Cytometry
Sample
Mouse Cell (Dorsal Root Ganglion (DRG) Cells E12)
Permeabilization
Yes - Saponin
Gating Strategy
1. FSC-A/SSC-A (exclusion of cell debris), 2. SSC-W (exclusion of cell doublets)
Specification
Dorsal Root Ganglion (DRG) Cells E12
Preparation
Cell harvesting/tissue preparation method: Trypsinization. Trituration.
Sample buffer: PBS BSA 1%
Fixation
Paraformaldehyde
Username

Georg Haase

Verified customer

Submitted Oct 14 2015

Thank you for your recent enquiry. Unfortunately, this antibody has not been tested for cross-reactivity with other family members. We apologise for the inconvenience caused, Please do not hesitate to contact us again if you need further inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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