Anti-GGA1 antibody (ab10551)
Key features and details
- Rabbit polyclonal to GGA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GGA1 antibody
See all GGA1 primary antibodies -
Description
Rabbit polyclonal to GGA1 -
Host species
Rabbit -
Specificity
The immunizing seqeunce is present only in isoform 1 of this protein, therefore cross-reactivity with splice variants is not expected. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
AMPTRPGEQASPEQPSA
, corresponding to amino acids 336-352 of Human GGA1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
Target
-
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
Post-translational
modificationsPhosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 26088 Human
- Omim: 606004 Human
- SwissProt: Q9UJY5 Human
- Unigene: 499158 Human
-
Alternative names
- 4930406E12Rik antibody
- ADP ribosylation factor binding protein 1 antibody
- ADP ribosylation factor binding protein GGA1 antibody
see all
Images
-
Anti-GGA1 antibody (ab10551) at 1/1350 dilution + Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/ml
Secondary
Goat-anti Rabbit IgG
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?The lysate prepared from HEK293 cells overexpressing YFP-GGA1 fusion protein. This accounts for the observed band of 100 kDa.
Datasheets and documents
-
Datasheet download
References (2)
ab10551 has been referenced in 2 publications.
- Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . PubMed: 17666033
- Wahle T et al. GGA1 is expressed in the human brain and affects the generation of amyloid beta-peptide. J Neurosci 26:12838-46 (2006). WB ; Human . PubMed: 17151287