Overview

  • Product nameAnti-GGA1 antibody
    See all GGA1 primary antibodies
  • Description
    Rabbit polyclonal to GGA1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH selected within first 50 aa of human GGA1.

  • Positive control
    • Mouse kidney tissue lysate. Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930406E12Rik antibody
    • ADP ribosylation factor binding protein 1 antibody
    • ADP ribosylation factor binding protein GGA1 antibody
    • ADP-ribosylation factor-binding protein GGA1 antibody
    • ARF binding protein 1 antibody
    • ARF-binding protein 1 antibody
    • Gamma adaptin related protein 1 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 1 antibody
    • GGA 1 antibody
    • GGA1 antibody
    • GGA1 protein antibody
    • GGA1_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 1 antibody
    • Golgi localized gamma ear containing ARF binding protein 1 antibody
    • Golgi-localized antibody
    • OTTHUMP00000028975 antibody
    • OTTHUMP00000042200 antibody
    see all

Anti-GGA1 antibody images

  • All lanes : Anti-GGA1 antibody (ab38454) at 1/100 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-GGA1 antibody (ab38454)

This product has been referenced in:
  • Kim H  et al. Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Nat Commun 5:5482 (2014). WB ; Mouse . Read more (PubMed: 25405894) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"