Overview

  • Product nameAnti-GGA1 antibody
    See all GGA1 primary antibodies
  • Description
    Mouse monoclonal to GGA1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: MEPAMEPETL EARINRATNP LNKELDWASI NGFCEQLNED FEGPPLATRL LAHKIQSPQE WEAIQALTVR RGEATIRPPP CDDTKGGQD, corresponding to amino acids 1-90 of Human GGA1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab57247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • FunctionPlays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • DomainThe VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930406E12Rik antibody
    • ADP ribosylation factor binding protein 1 antibody
    • ADP ribosylation factor binding protein GGA1 antibody
    • ADP-ribosylation factor-binding protein GGA1 antibody
    • ARF binding protein 1 antibody
    • ARF-binding protein 1 antibody
    • Gamma adaptin related protein 1 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 1 antibody
    • GGA 1 antibody
    • GGA1 antibody
    • GGA1 protein antibody
    • GGA1_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 1 antibody
    • Golgi localized gamma ear containing ARF binding protein 1 antibody
    • Golgi-localized antibody
    • OTTHUMP00000028975 antibody
    • OTTHUMP00000042200 antibody
    see all

Anti-GGA1 antibody images



  • Predicted band size : 10 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GGA1 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab57247 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab57247 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab57247 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • GGA1 antibody (ab57247) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 10 kDa
    GGA1 antibody (ab57247) at 1ug/lane + K-562 cell lysate at 25ug/lane.

References for Anti-GGA1 antibody (ab57247)

ab57247 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"