Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Belongs to the GGA protein family. Contains 1 GAE domain. Contains 1 GAT domain. Contains 1 VHS domain.
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif). The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis. The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs. The GAE domain binds accessory proteins regulating GGAs function.
Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated.
Golgi localized gamma ear containing ARF binding protein 1 antibody
Western blot - Anti-GGA1 antibody [EPR3014(2)] (ab170956)
Predicted band size : 70 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: GGA1 knockout HAP1 cell lysate (20 µg) Lane 3: Jurkat cell lysate (20 µg) Lane 4: HeLa cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab170956 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa. ab170956 was shown to recognize GGA1 when GGA1 knockout samples were used, along with additional cross-reactive bands. Wild-type and GGA1 knockout samples were subjected to SDS-PAGE. ab170956 and ab8245 (loading control to GAPDH) were diluted 2 µg/ml and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.