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Our Abpromise guarantee covers the use of ab10552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).|
Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"