Overview

  • Product name
  • Description
    Rabbit polyclonal to GGA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 20-280 of Human GGA2 (UniProt: Q9UJY4).

  • Positive control
    • HepG2, MOLT4 and Raji whole cell lysates; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 67 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200007E24Rik antibody
    • ADP ribosylation factor binding protein 2 antibody
    • ADP ribosylation factor binding protein GGA 2 antibody
    • ADP ribosylation factor binding protein GGA2 antibody
    • ADP-ribosylation factor-binding protein GGA2 antibody
    • ARF-binding protein 2 antibody
    • FLJ20966 antibody
    • Gamma adaptin related protein 2 antibody
    • gamma ear-containing antibody
    • Gamma-adaptin-related protein 2 antibody
    • GGA 2 antibody
    • Gga2 antibody
    • GGA2_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 2 antibody
    • Golgi localized gamma ear containing ARF binding protein 2 antibody
    • Golgi-localized antibody
    • KIAA1080 antibody
    • Vear antibody
    • VHS domain and ear domain containing protein antibody
    • VHS domain and ear domain of gamma adaptin antibody
    • VHS domain and ear domain of gamma-adaptin antibody
    see all

Images

  • Anti-GGA2 antibody (ab153928) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 67 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HepG2 cells, labeling GGA2 with ab153928 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab153928 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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