Recombinant
RabMAb

Anti-GGA3 antibody [EPR14340-9] (ab182005)

Overview

  • Product name
    Anti-GGA3 antibody [EPR14340-9]
    See all GGA3 primary antibodies
  • Description
    Rabbit monoclonal [EPR14340-9] to GGA3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GGA3 aa 400-550 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NZ52

  • Positive control
    • A431 and 293 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14340-9
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab182005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).

Target

  • Function
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding and an autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • Post-translational
    modifications
    Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
    Ubiquitinated.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor binding protein 3 antibody
    • ADP ribosylation factor binding protein GGA 3 antibody
    • ADP ribosylation factor binding protein GGA3 antibody
    • ADP-ribosylation factor-binding protein GGA3 antibody
    • ARF binding protein GGA 3 antibody
    • ARF binding protein GGA3 antibody
    • ARF-binding protein 3 antibody
    • gamma ear-containing antibody
    • GGA 3 antibody
    • GGA3 antibody
    • GGA3_HUMAN antibody
    • Golgi associated gamma adaptin ear containing ARF binding protein 3 antibody
    • Golgi localized gamma ear containing ARF binding protein 3 antibody
    • Golgi-localized antibody
    • KIAA0154 antibody
    see all

Images

  • Anti-GGA3 antibody [EPR14340-9] (ab182005) at 1/50000 dilution + 293 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size : 78 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Anti-GGA3 antibody [EPR14340-9] (ab182005) at 1/5000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size : 78 kDa

References

ab182005 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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