Anti-Ghrelin antibody [45] (ab112477)
Key features and details
- Mouse monoclonal [45] to Ghrelin
- Suitable for: ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Ghrelin antibody [45]
See all Ghrelin primary antibodies -
Description
Mouse monoclonal [45] to Ghrelin -
Host species
Mouse -
Specificity
ab112477 binds Human ghrelin and Human des-acyl ghrelin, cross-reacting ~5% with rat ghrelin (determined by sandwich ELISA). -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Ghrelin. Synthetic Human Ser3-octanoyl ghrelin-28
Database link: Q9UBU3-1 -
Epitope
Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 97% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
45 -
Isotype
IgG1 -
Light chain type
lambda -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Target
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Function
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. -
Tissue specificity
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. -
Sequence similarities
Belongs to the motilin family. -
Post-translational
modificationsO-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 51738 Human
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
- Unigene: 590080 Human
- Unigene: 672979 Human
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Alternative names
- Appetite regulating hormone antibody
- Ghrelin 27 antibody
- Ghrelin 28 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab112477 has been referenced in 2 publications.
- Kojima M & Kangawa K Ghrelin: structure and function. Physiol Rev 85:495-522 (2005). PubMed: 15788704
- Kojima M et al. Ghrelin: discovery of the natural endogenous ligand for the growth hormone secretagogue receptor. Trends Endocrinol Metab 12:118-22 (2001). PubMed: 11306336